POSSIBLE ERRORS IN ASSAY FOR BETA-GLYCOSIDASE ACTIVITY

被引:8
作者
CHADWICK, RW [1 ]
ALLISON, JC [1 ]
TALLEY, DL [1 ]
GEORGE, SE [1 ]
机构
[1] US EPA,DIV GENET TOXICOL,HLTH EFFECTS RES LAB MD 68,RES TRIANGLE PK,NC 27711
关键词
D O I
10.1128/AEM.61.2.820-822.1995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cecal homogenates were assayed for the enzymes beta-glucosidase, beta-glucuronidase, and beta-galactosidase. Anaerobic incubation with the addition of excess 3,4-dichloronitrobenzene, a substrate for nitroreductase, significantly increased the detection of the beta-glycosidase enzymes' activities.
引用
收藏
页码:820 / 822
页数:3
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