THE ESR PROPERTIES, DNA NICKING, AND DNA ASSOCIATION OF AGED SOLUTIONS OF CATECHOL VERSUS AQUEOUS EXTRACTS OF TAR FROM CIGARETTE-SMOKE

被引：53

作者：

STONE, K ^{[1
]}

BERMUDEZ, E ^{[1
]}

ZANG, LY ^{[1
]}

CARTER, KM ^{[1
]}

QUEENAN, KE ^{[1
]}

PRYOR, WA ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV, INST BIODYNAM, BATON ROUGE, LA 70803 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 319卷 / 01期

关键词：

SEMIQUINONE RADICALS; AUTOXIDATION; HYDROGEN PEROXIDE; CATALASE; SUPEROXIDE DISMUTASE; GLUTATHIONE; CANCER; ENVIRONMENTAL TOBACCO SMOKE;

D O I：

10.1006/abbi.1995.1282

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies in our laboratories have shown that extracts from mainstream or sidestream tobacco smoke nick DNA. These solutions contain the tar semiquinone free radical, and the tar radical becomes associated with cellular DNA. Aged solutions of catechol contain a semiquinone species that has ESR properties similar to those of the radical in cigarette tar extracts, and we have used these catechol solutions as a model for the tar radical. Both the radical in aged catechol solutions and the cigarette tar radical become associated with the DNA in mammalian cells and also nick DNA. The nicking of DNA caused by both tar and aged catechol solutions follows saturation kinetics. Aged catechol solutions thus allow the study of a model for the radical present in cigarette tar, without interference from the other toxic constituents in tar extracts. (C) 1995 Academic Press, Inc.

引用

页码：196 / 203

页数：8

共 54 条

[1] GLUTATHIONE-DEPENDENT TOXICITY [J].

ANDERS, MW ;

DEKANT, W ;

VAMVAKAS, S .

XENOBIOTICA, 1992, 22 (9-10) :1135-1145

[2] ENVIRONMENTAL TOBACCO-SMOKE IS JUST AS DAMAGING TO DNA AS MAINSTREAM SMOKE [J].

BERMUDEZ, E ;

STONE, K ;

CARTER, KM ;

PRYOR, WA .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 (10) :870-874

[3]

BIRNBOIM HC, 1990, METHOD ENZYMOL, V186, P550

[4] CIGARETTE TAR CAUSES SINGLE-STRAND BREAKS IN DNA [J].

BORISH, ET ;

COSGROVE, JP ;

CHURCH, DF ;

DEUTSCH, WA ;

PRYOR, WA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 133 (02) :780-786

[5] DNA-SYNTHESIS IS BLOCKED BY CIGARETTE TAR-INDUCED DNA SINGLE-STRAND BREAKS [J].

BORISH, ET ;

PRYOR, WA ;

VENUGOPAL, S ;

DEUTSCH, WA .

CARCINOGENESIS, 1987, 8 (10) :1517-1520

[6]

BORISH ET, 1986, SUPEROXIDE SUPEROXID, P467

[7] REACTIONS OF GLUTATHIONE AND GLUTATHIONE RADICALS WITH BENZOQUINONES [J].

BUTLER, J ;

HOEY, BM .

FREE RADICAL BIOLOGY AND MEDICINE, 1992, 12 (05) :337-345

[8] FREE-RADICAL CHEMISTRY OF CIGARETTE-SMOKE AND ITS TOXICOLOGICAL IMPLICATIONS [J].

CHURCH, DF ;

PRYOR, WA .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1985, 64 :111-126

[9] THE METAL-MEDIATED FORMATION OF HYDROXYL RADICAL BY AQUEOUS EXTRACTS OF CIGARETTE TAR [J].

COSGROVE, JP ;

BORISH, ET ;

CHURCH, DF ;

PRYOR, WA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 132 (01) :390-396

[10]

DAVIS WB, 1988, J LAB CLIN MED, V111, P293

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