GEL FILTRATION OF MITOCHONDRIAL MEMBRANE PROTEINS DISSOLVED IN CHLOROFORM-METHANOL

被引：17

作者：

CURTIS, PJ

机构：

[1] The Beatson Institute for Cancer Research, Royal Beatson Memorial Hospital, Glasgow

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 194卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0005-2795(69)90112-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The use of chloroform-methanol (2:1, v/v) as solvent for mitochondrial membrane protein has been investigated. 2. 2. Sephadex G-75, G-100, G-200 after exhaustive methylation swell readily in chloroform-methanol and still act as a molecular sieve. 3. 3. Gel filtration, using methylated Sephadex G-100 in chloroform-methanol, achieved a separation of the main components of mitochondrial membrane protein as judged by polyacrylamide gel electrophoresis. © 1969.

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页码：513 / &

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