RAPID DETECTION OF HOG-CHOLERA VIRUS IN TISSUES BY THE POLYMERASE CHAIN-REACTION

被引：52

作者：

LIU, ST ^{[1
]}

LI, SN ^{[1
]}

WANG, DC ^{[1
]}

CHANG, SF ^{[1
]}

CHIANG, SC ^{[1
]}

HO, WC ^{[1
]}

CHANG, YS ^{[1
]}

LAI, SS ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,DEPT VET MED,TAIPEI,TAIWAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 35卷 / 02期

关键词：

HOG CHOLERA VIRUS; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0166-0934(91)90138-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method for the detection of hog cholera virus (HCV) in infected tissues, using polymerase chain reaction (PCR) was developed. Total RNA isolated from HCV-infected tissues was reverse transcribed with AMV reverse transcriptase and the resulting complementary DNA was amplified by Taq DNA polymerase in the presence of two HCV-specific primers. The amplified DNA fragment was detected by agarose gel electrophoresis. The sensitivity of this method was at 10(4) TCID50 of HCV. The sensitivity increased approximately 1000-fold when the DNA was reamplified with a set of nested primers. DNA sequencing analysis of the PCR products revealed that the HCV sequence amplified from a local field isolate was highly homologous to the HCV Alfort strain. This method may be useful for pathological and epidemiological studies of HCV in pigs.

引用

页码：227 / 236

页数：10

共 19 条

[1] NUCLEOTIDE-SEQUENCE OF THE ACEB GENE ENCODING MALATE SYNTHASE-A IN ESCHERICHIA-COLI [J].