REVISED SEQUENCE AND ACTIVITY OF BACILLUS-STEAROTHERMOPHILUS CATALASE-I (FORMERLY PEROXIDASE)

被引：10

作者：

TRAKULNALEAMSAI, S ^{[1
]}

AIHARA, S ^{[1
]}

MIYAI, K ^{[1
]}

SUGA, Y ^{[1
]}

SOTA, M ^{[1
]}

YOMO, T ^{[1
]}

URABE, I ^{[1
]}

机构：

[1] OSAKA UNIV, FAC ENGN, DEPT BIOTECHNOL, 2-1 YAMADAOKA, SUITA, OSAKA 565, JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 04期

关键词：

D O I：

10.1016/0922-338X(92)90117-D

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The revised nucleotide sequence of the perA gene, encoding catalase I (formerly peroxidase) of Bacillus stearothermophilus, is presented. The deduced amino acid sequence was significantly similar to those of Escherichia coli catalase HPI, Salmonella typhimurium catalase HPI, and yeast cytochrome c peroxidase. Catalase I was purified and the ratio of the catalatic to peroxidatic activity was measured and found to be 654 to 1.

引用

页码：234 / 237

页数：4

共 15 条

[1]

AEBI HE, 1983, METHODS ENZYME ANAL, V3, P273

[2]

CLAIBORNE A, 1979, J BIOL CHEM, V254, P4245

[3]

HINDERBRANT AG, 1975, ARCH BIOCHEM BIOPHYS, V171, P385

[4]

KAPUT J, 1982, J BIOL CHEM, V257, P5054

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] NUCLEOTIDE-SEQUENCE OF KATG OF SALMONELLA-TYPHIMURIUM LT2 AND CHARACTERIZATION OF ITS PRODUCT, HYDROPEROXIDASE-I [J].

LOEWEN, PC ;

STAUFFER, GV .

MOLECULAR AND GENERAL GENETICS, 1990, 224 (01) :147-151

[7]

LOPRASERT S, 1988, J GEN MICROBIOL, V134, P1971

[8] CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION IN ESCHERICHIA-COLI OF THE BACILLUS-STEAROTHERMOPHILUS PEROXIDASE GENE (PERA) [J].

LOPRASERT, S ;

NEGORO, S ;

OKADA, H .

JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4871-4875

[9] OVERPRODUCTION AND SINGLE-STEP PURIFICATION OF BACILLUS-STEAROTHERMOPHILUS PEROXIDASE IN ESCHERICHIA-COLI [J].

LOPRASERT, S ;

URABE, I ;

OKADA, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 32 (06) :690-692

[10]

Michal G, 1983, METHOD ENZYMAT AN, V1, P197

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