TYROSINATABLE AND NON-TYROSINATABLE TUBULIN SUBPOPULATIONS IN RAT MUSCLE IN COMPARISON WITH THOSE IN BRAIN

被引:11
作者
ALONSO, AD [1 ]
ARCE, CA [1 ]
BARRA, HS [1 ]
机构
[1] NATL UNIV CORDOBA,FAC CIENCIAS QUIM,CTR INVEST QUIM BIOL CORDOBA,DEPT QUIM BIOL,CIUDAD UNIV,RA-5016 CORDOBA,ARGENTINA
关键词
TUBULIN; DETYROSINATION; CARBOXYPEPTIDASE; (BRAIN); (MUSCLE);
D O I
10.1016/0167-4838(93)90274-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using immunobinding and enzymatic assays we determined in rat muscle extracts the proportion of tyrosinatable tubulin, that is, tubulin that participates in the tyrosination/detyrosination cycle. We found that in muscle, in contrast with nervous tissue, practically all tubulin molecules are tyrosinatable. In the case of rat brain the non-tyrosinatable tubulin pool accounts for about 50% of the tubulin. In addition, isoelectrofocusing of C-14-tyrosinated tubulin from brain and muscle extracts revealed a different composition in tyrosinatable tubulin isotypes. One of the isotypes, which in muscle accounts for 86% of the C-14-tyrosinated tubulin species, was detyrosinated by the action of tubulin carboxypeptidase faster than the rest of the C-14-tyrosinated tubulin isotypes taken in whole. In the case of brain extract, that isotype accounts for only 16% of the labeled tubulin.
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页码:26 / 30
页数:5
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