A SIMPLE APPARATUS FOR MEASURING THE EH OF ANAEROBIC MEDIA

被引:11
作者
PATEL, GB [1 ]
AGNEW, BJ [1 ]
机构
[1] NATL RES COUNCIL CANADA, DIV BIOL SCI, OTTAWA K1A 0R6, ONTARIO, CANADA
关键词
D O I
10.1139/m81-134
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 22-mm outside diameter screw cap test tube was modified for measuring the redox potential (Eh) of samples of anaerobic media. The screw cap was fitted with a grey butyl rubber septum with a hole through which a platinum-calomel combination electrode was inserted. The tube was shortened in length and a small side arm that could be sealed with a serum stopper and an aluminium seal was attached. This device enabled the measurement of Eh of anaerobic media without the need for continuous flushing of the vessel headspace to maintain anaerobiosis.
引用
收藏
页码:853 / 855
页数:3
相关论文
共 7 条
[1]  
HOLDEMAN LV, 1975, ANAEROBE LABORATORY, P1
[2]  
Jacob H. E., 1970, METHODS MICROBIOL, V2, P91, DOI 10.1016/S0580-9517(08)70218-6
[3]   SERUM BOTTLE MODIFICATION OF HUNGATE TECHNIQUE FOR CULTIVATING OBLIGATE ANAEROBES [J].
MILLER, TL ;
WOLIN, MJ .
APPLIED MICROBIOLOGY, 1974, 27 (05) :985-987
[4]   CHARACTERIZATION OF A STRAIN OF METHANOSPIRILLUM-HUNGATII [J].
PATEL, GB ;
ROTH, LA ;
VANDENBERG, L ;
CLARK, DS .
CANADIAN JOURNAL OF MICROBIOLOGY, 1976, 22 (09) :1404-1410
[5]   EFFECT OF SODIUM-CHLORIDE ON GROWTH AND METHANE PRODUCTION OF METHANOGENS [J].
PATEL, GB ;
ROTH, LA .
CANADIAN JOURNAL OF MICROBIOLOGY, 1977, 23 (07) :893-897
[6]   ISOLATION AND CHARACTERIZATION OF METHANOBACTERIUM-RUMINANTIUM N-SP [J].
SMITH, PH ;
HUNGATE, RE .
JOURNAL OF BACTERIOLOGY, 1958, 75 (06) :713-718
[7]  
1966, B ST RADIOMETER AS, V40