ANALYSIS OF RAT-BRAIN MICRODIALYSATE BY GAS-CHROMATOGRAPHY HIGH-RESOLUTION SELECTED-ION MONITORING MASS-SPECTROMETRY

被引：8

作者：

CHANG, SY ^{[1
]}

MOORE, TA ^{[1
]}

DEVAUD, LL ^{[1
]}

TAYLOR, LCE ^{[1
]}

HOLLINGSWORTH, EB ^{[1
]}

机构：

[1] BURROUGHS WELLCOME CO,3030 CORNWALLIS RD,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80569-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gas chromatography-high-resolution selected-ion monitoring mass spectrometry was used to analyze catecholamine metabolites in rat brain microdialysate. Dialysate samples were collected in vials containing stable isotope analogues of homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG) and 5-hydroxyindoleacetic acid (5HIAA) and analyzed as their trimethylsilyl derivatives. The metabolite levels were monitored at 20-min intervals throughout the time course of the experiment, beginning immediately after surgery and implantation of the dialysis probe and ending 4 h after amphetamine treatment. The levels of HVA were observed to decrease after amphetamine treatment, while those of MHPG and 5HIAA did not change significantly.

引用

页码：111 / 118

页数：8

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