SOMATIC HYBRID PLANTS FROM SEXUALLY INCOMPATIBLE WOODY SPECIES - CITRUS-RETICULATA AND CITROPSIS-GILLETIANA

被引：43

作者：

GROSSER, JW

GMITTER, FG

TUSA, N

CHANDLER, JL

机构：

[1] Citrus Research and Education Center, University of Florida, IFAS, Lake Alfred, 33850, FL

[2] Centro di Studio del C.N.R. per il Miglioramento Genetico degli Agrumi, Palermo, I-90128, Viale delle Scienze

来源：

PLANT CELL REPORTS | 1990年 / 8卷 / 11期

关键词：

D O I：

10.1007/BF00269986

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Allotetraploid intergeneric somatic hybrid plants between Citrus reticulata Blanco cv. Cleopatra mandarin and Citropsis gilletiana Swing. & M. Kell. (common name Gillet's cherry orange) were regenerated following protoplast fusion. Cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of Citropsis gilletiana. Cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. Hybrid plant identification was based on differential leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose mutase (PGM) and phosphohexose isomerase (PHI) isozyme banding patterns. This is the first somatic hybrid within the Rutaceae reported that does not have Citrus sinensis (sweet orange) as a parent, and the first produced with a commercially important citrus rootstock and a complementary but sexually incompatible, related species. © 1990 Springer-Verlag.

引用

页码：656 / 659

页数：4

共 19 条

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[2]

CASTLE WS, 1987, ROOTSTOCKS FRUITS CR, pCH11

[3]

Ford H. W., 1960, Proceedings of the Florida State Horticultural Society held at Tampa, Florida, October 25-27, 1960., V73, P60

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