ENZYME-CATALYZED SYNTHESIS OF ALKYL BETA-D-GLYCOSIDES WITH CRUDE HOMOGENATE OF SULFOLOBUS-SOLFATARICUS

被引：42

作者：

TRINCONE, A ^{[1
]}

NICOLAUS, B ^{[1
]}

LAMA, L ^{[1
]}

MORZILLO, P ^{[1
]}

DEROSA, M ^{[1
]}

GAMBACORTA, A ^{[1
]}

机构：

[1] UNIV NAPLES,IST BIOCHIM MACROMOLEC,I-80138 NAPLES,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 04期

关键词：

D O I：

10.1007/BF01041476

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Crude homogenate of thermophilic archaebacterium Sulfolobus solfataricus, possessing a beta-glycosidase, has been used to synthesize different alkyl beta-D-glycosides starting from phenyl beta-D-glucoside, phenyl beta-D-galactoside and lactose as carbohydrate donors. High product yield (95% with respect to the carbohydrate donor) of octyl beta-D-glucoside has been obtained in a two-phase system containing 5% of water. The enantioselection for the galactosyl transfer to the secondary hydroxyl group of propane-1,2-diol is higher than that found using beta-galactosidase from E. coli.

引用

页码：235 / 240

页数：6

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