PRODUCTION OF LIPID HYDROPEROXIDES AND DEPLETION OF REDUCED GLUTATHIONE IN LIVER-MITOCHONDRIA AFTER ACUTE ETHANOL ADMINISTRATION TO RATS

被引：16

作者：

TRENTI, T

STERNIERI, E

CECCARELLI, D

GALLESI, D

MASINI, A

机构：

[1] UNIV MODENA,IST PATOL GEN,VIA CAMPI 287,I-41100 MODENA,ITALY

[2] UNIV MODENA,INST CLIN PHARMACOL & TOXICOL,I-41100 MODENA,ITALY

来源：

TOXICOLOGY LETTERS | 1992年 / 64-5卷

关键词：

ETHANOL; REDUCED GLUTATHIONE; LIPID HYDROPEROXIDES; RAT LIVER MITOCHONDRIA;

D O I：

10.1016/0378-4274(92)90257-K

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

It bas been found that acute ethanol (EtOH) intoxication to rats caused approximately 40% depletion of mitochondrial reduced glutathione (GSH). A GSH reduction of similar extent was also observed after the administration to rats of buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis. The combined treatment of EtOH plus BSO induced a further mitochondrial GSH decrease up to 70% with respect to control. The presence of lipid hydroperoxides in the mitochondrial membrane was observed whenever an additional oxidative stress was associated to a condition of GSH depletion as in the case of EtOH or EtOH plus BSO. Under these conditions a severe derangement in mitochondrial oxidative functions occurred.

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页码：751 / 755

页数：5

相关论文

共 10 条

[1] CONJUGATED DIENE AND TRANS-FATTY-ACIDS IN TISSUE-LIPIDS OF RATS FED AN HEPATOCARCINOGENIC CHOLINE-DEVOID DIET [J].

BANNI, S ;

SALGO, MG ;

EVANS, RW ;

CORONGIU, FP ;

LOMBARDI, B .

CARCINOGENESIS, 1990, 11 (11) :2053-2057

[2] CARBON-TETRACHLORIDE, BROMOTRICHLOROMETHANE AND ETHANOL ACUTE INTOXICATION - NEW CHEMICAL EVIDENCE FOR LIPID-PEROXIDATION IN RAT-TISSUE MICROSOMES [J].

CORONGIU, FP ;

LAI, M ;

MILIA, A .

BIOCHEMICAL JOURNAL, 1983, 212 (03) :625-631

[3] IMPAIRED UPTAKE OF GLUTATHIONE BY HEPATIC MITOCHONDRIA FROM CHRONIC ETHANOL-FED RATS - TRACER KINETIC-STUDIES INVITRO AND INVIVO AND SUSCEPTIBILITY TO OXIDANT STRESS [J].

FERNANDEZCHECA, JC ;

GARCIARUIZ, C ;

OOKHTENS, M ;

KAPLOWITZ, N .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (02) :397-405

[4] LIPID PEROXIDATION - MECHANISM INVOLVED IN ACUTE ETHANOL TOXICITY AS DEMONSTRATED BY INVIVO PENTANE PRODUCTION IN RAT [J].

LITOV, RE ;

IRVING, DH ;

DOWNEY, JE ;

TAPPEL, AL .

LIPIDS, 1978, 13 (04) :305-307

[5] MITOCHONDRIAL INNER MEMBRANE-PERMEABILITY CHANGES INDUCED BY OCTADECADIENOIC ACID HYDROPEROXIDE - ROLE OF MITOCHONDRIAL GSH POOL [J].

MASINI, A ;

CECCARELLI, D ;

TRENTI, T ;

GALLESI, D ;

MUSCATELLO, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1101 (01) :84-89

[6] PERTURBATION IN LIVER MITOCHONDRIAL CA-2+ HOMEOSTASIS IN EXPERIMENTAL IRON OVERLOAD - A POSSIBLE FACTOR IN CELL INJURY [J].

MASINI, A ;

CECCARELLI, D ;

TRENTI, T ;

CORONGIU, FP ;

MUSCATELLO, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1014 (02) :133-140

[7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF NANOMOLE LEVELS OF GLUTATHIONE, GLUTATHIONE DISULFIDE, AND RELATED THIOLS AND DISULFIDES [J].

REED, DJ ;

BABSON, JR ;

BEATTY, PW ;

BRODIE, AE ;

ELLIS, WW ;

POTTER, DW .

ANALYTICAL BIOCHEMISTRY, 1980, 106 (01) :55-62

[8] OXIDATIVE STRESS IN MITOCHONDRIA - ITS RELATIONSHIP TO CELLULAR CA-2+ HOMEOSTASIS, CELL-DEATH, PROLIFERATION, AND DIFFERENTIATION [J].

RICHTER, C ;

KASS, GEN .

CHEMICO-BIOLOGICAL INTERACTIONS, 1991, 77 (01) :1-23

[9] INCREASED LOSS AND DECREASED SYNTHESIS OF HEPATIC GLUTATHIONE AFTER ACUTE ETHANOL ADMINISTRATION - TURNOVER STUDIES [J].

SPEISKY, H ;

MACDONALD, A ;

GILES, G ;

ORREGO, H ;

ISRAEL, Y .

BIOCHEMICAL JOURNAL, 1985, 225 (03) :565-572

[10] EFFECT OF ACUTE ETHANOL INTOXICATION ON THE CONTENT OF REDUCED GLUTATHIONE OF THE LIVER IN RELATION TO ITS LIPOPEROXIDATIVE CAPACITY IN THE RAT [J].

VIDELA, LA ;

FERNANDEZ, V ;

UGARTE, G ;

VALENZUELA, A .

FEBS LETTERS, 1980, 111 (01) :6-10