QUALITATIVE AND QUANTITATIVE-DETERMINATION OF TAXOL AND RELATED-COMPOUNDS IN TAXUS-CUSPIDATA SIEB ET ZUCC

被引：35

作者：

FANG, WH

WU, Y

ZHOU, JY

CHEN, WM

FANG, QC

机构：

[1] Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing

来源：

PHYTOCHEMICAL ANALYSIS | 1993年 / 4卷 / 03期

关键词：

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TAXOL; TAXUS-CUSPIDATA; TAXUS SPECIES;

D O I：

10.1002/pca.2800040307

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper the determination of taxol and the related compounds 10-deacetyltaxol, cephalomannine and 10-deacetylcephalomannine in various parts of Taxus cuspidata Sieb et Zucc. is reported. All four compounds were well separated on a 5 mum Spheri-5 RP-18 column with a mobile phase composed of water:acetonitrile:tetrahydrofuran (55:35:10). The concentrations of these compounds are quite different for different parts of the plant and are highest in stem bark. Furthermore, the content is related to the age of the plant, the older plants containing larger quantities of taxane diterpenoids. We have also found that the contents of taxol and related compounds in the shrub are usually higher than those in the tree.

引用

页码：115 / 119

页数：5

共 12 条

[1]

Harvey S.D., Campbell J.A., Kelsey R.G., Vance N.C., Separation of taxol from related taxanes in Taxus brevifolia extracts by isocratic elution reversed‐phase microcolumn high performance liquid chromatography, J. Chromatogr., 587, pp. 300-305, (1991)

[2]

High performance liquid chromatography, Analytical Chemistry, pp. 473-495, (1979)

[3]

Schiff P.B., Fant J., Horwitz S.B., Promotion of microtubule assembly in vitro by taxol, Nature, 277, pp. 665-667, (1979)

[4]

Schiff P.B., Horwitz S.B., Taxol stabilizes micro‐tubules in mouse fibroblast cells, Proc. Natl Acad. Sci., 77, pp. 1561-1565, (1980)

[5]

Stasko M.W., Witherup K.M., Ghiorzi T.J., McCloud T.G., Look S., Muschik G.M., Issaq H.J., Multimodal thin layer chromatographic separation of taxol and related compounds from Taxus brevifolia, J. Liq. Chromatogr., 12, pp. 2133-2143, (1989)

[6]

Vedensek N., Lim P., Campbell A., Carlson C., Taxol content in bark, wood, root, leaf, twig, and seedling from several Taxus species, Journal of Natural Products, 53, pp. 1609-1610, (1990)

[7]

Wani M.C., Taylor H.L., Wall M.E., Coggon P., McPhail A.T., Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia, J. Am. Chem. Soc., 93, pp. 2325-2327, (1971)

[8]

Wheeler N.C., Jech K., Masters S., Brobst S.W., Alvarado A.B., Hoover A.J., Snader K.M., Effects of genetic, epigenetic and environmental factors on taxol content in Taxus brevifolia and related species, J. Nat. Prod., 55, pp. 432-440, (1992)

[9]

Witherup K.M., Look S.A., Stasko M.W., McCloud T.G., Issaq H.J., Muschik G.M., High performance chromatographic separation of taxol and related compounds from Taxus brevifolia, J. Liq. Chromatogr., 12, pp. 2117-2132, (1989)

[10]

Witherup K.M., Look S.A., Stasko M.W., Ghiorzi T.J., Muschik G.M., Taxus spp. needles contain amounts of taxol and comparable to the bark of Taxus brevifolia: analysis and isolation, J. Nat. Prod., 53, pp. 1249-1255, (1990)

← 1 2 →