CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF HUMAN BETA-GLUCURONIDASE

被引：4

作者：

DRENDEL, WB

GRUBB, JH

SLY, WS

CHEN, ZW

MATHEWS, FS

JAIN, S

机构：

[1] ST LOUIS UNIV, SCH MED, EA DOISY DEPT BIOCHEM & MOLEC BIOL, 1402 S GRAND BLVD, ST LOUIS, MO 63104 USA

[2] WASHINGTON UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOPHYS, ST LOUIS, MO 63110 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 01期

关键词：

ACID HYDROLASES; EXOGLYCOSIDASES; LYSOSOMAL ENZYMES; MUCOPOLYSACCHARIDOSIS; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1993.1494

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of human β-glucuronidase have been obtained by the vapor diffusion method, using 2-methyl-2,4-pentanediol as a precipitant. The crystals belong to the orthorhombic space group P2221, with cell dimensions a=134.5 Å, b=95.1 Å, c=124.4 Å. The unit cell contains two copies of the tetrameric enzyme. Complete native data have been collected to a resolution of 2.6 Å. © 1993 Academic Press Limited.

引用

页码：173 / 176

页数：4

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