NEW AND SPECIFIC NUCLEOSIDE DIPHOSPHATE GLUCOSE SUBSTRATES FOR GLYCOGENIN

被引：16

作者：

ALONSO, MD ^{[1
]}

LAGZDINS, EJ ^{[1
]}

LOMAKO, J ^{[1
]}

LOMAKO, WM ^{[1
]}

WHELAN, WJ ^{[1
]}

机构：

[1] UNIV MIAMI,SCH MED M823,DEPT BIOCHEM & MOLEC BIOL,MIAMI,FL 33101

来源：

FEBS LETTERS | 1995年 / 359卷 / 2-3期

关键词：

GLYCOGENIN; APO-GLYCOGENIN; CDP-GLUCOSE; TDP-GLUCOSE;

D O I：

10.1016/0014-5793(95)00018-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycogenin, the autocatalytic, self-glucosylating primer for glycogen synthesis by glycogen synthase, is presumed, in vivo, to use UDP-glucose as the source of the glucose residues it adds to itself, When we tested its ability to utilize other nucleoside diphosphate glucoses, it emerged that purine nucleotides are not utilized but two pyrimidine nucleotides are used, in addition to UDP-glucose, These are CDP-glucose and TDP-glucose. CDP-glucose is utilized at 70% of the rate of UDP-glucose. While there is no evidence that CDP-glucose is a natural substrate for glycogenin, it has the advantage over UDP-glucose in that it can be used specifically to detect and assay glycogenin in the presence of glycogen synthase because CDP-glucose, unlike UDP-glucose, is not a substrate for the synthase.

引用

页码：110 / 112

页数：3

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