CRYOPRESERVATION OF SPORES OF VESICULAR ARBUSCULAR MYCORRHIZAL FUNGI

被引：24

作者：

DOUDS, DD ^{[1
]}

SCHENCK, NC ^{[1
]}

机构：

[1] UNIV FLORIDA, DEPT PLANT PATHOL, GAINESVILLE, FL 32611 USA

来源：

NEW PHYTOLOGIST | 1990年 / 115卷 / 04期

关键词：

cryopreservation; germination; VA mycorrhizal fungi;

D O I：

10.1111/j.1469-8137.1990.tb00498.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Storage of spores of vesicular‐arbuscular (VA) mycorrhizal fungi in soil at 5 °C is a common way of preserving these fungi. This method was satisfactory for Glomus intraradix Schenck & Smith but not for Gigaspora margarita Becker & Hall, Glomus mosseae (Nicol. & Gerd.) Gerdemann & Trappe, and Acaulospora longula Spain & Schenck. Preservation of spores at −60 to −70 °C was examined. Cryoprotectants such as DMSO, glycerol, mannitol, and sucrose were ineffective using the freeze‐damage sensitive species G. margarita. Incubation for 47 h in 0.75 to 1.0 M trehalose conferred a measure of freeze damage protection to the spores such that germination rates of previously frozen spores of G. margarita were one tenth to one sixth of controls. The best method of cryoprotection and cryopreservation was found to be slow drying of pot culture soil and freezing the spores in situ. This procedure was satisfactory for the five genera of VA mycorrhizal fungi evaluated. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：667 / 674

页数：8

共 23 条

[1] EFFECT OF A FREEZE-THAW CYCLE ON PROPERTIES OF MICROSOMAL-MEMBRANES FROM WHEAT [J].

BOROCHOV, A ;

WALKER, MA ;

KENDALL, EJ ;

PAULS, KP ;

MCKERSIE, BD .

PLANT PHYSIOLOGY, 1987, 84 (01) :131-134

[2] LIPID-SUGAR INTERACTIONS - RELEVANCE TO ANHYDROUS BIOLOGY [J].

CAFFREY, M ;

FONSECA, V ;

LEOPOLD, AC .

PLANT PHYSIOLOGY, 1988, 86 (03) :754-758

[3] INFRARED SPECTROSCOPIC STUDIES ON INTERACTIONS OF WATER AND CARBOHYDRATES WITH A BIOLOGICAL MEMBRANE [J].

CROWE, JH ;

CROWE, LM ;

CHAPMAN, D .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1984, 232 (01) :400-407

[4] EFFECTS OF CARBOHYDRATES ON MEMBRANE STABILITY AT LOW WATER ACTIVITIES [J].

CROWE, LM ;

MOURADIAN, R ;

CROWE, JH ;

JACKSON, SA ;

WOMERSLEY, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 769 (01) :141-150

[5] PREVENTION OF FUSION AND LEAKAGE IN FREEZE-DRIED LIPOSOMES BY CARBOHYDRATES [J].

CROWE, LM ;

WOMERSLEY, C ;

CROWE, JH ;

REID, D ;

APPEL, L ;

RUDOLPH, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 861 (01) :131-140

[6] INFECTIVITY OF VESICULAR ARBUSCULAR MYCORRHIZAL INOCULA AFTER STORAGE UNDER VARIOUS ENVIRONMENTAL-CONDITIONS [J].

DAFT, MJ ;

SPENCER, D ;

THOMAS, GE .

TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1987, 88 :21-27

[7]

DALPE Y, 1987, 7TH P N AM C MYC GAI, P279

[8] CONSERVATION OF FUNGOUS CULTURES [J].

FENNELL, DI .

BOTANICAL REVIEW, 1960, 26 (01) :79-141

[9]

Ferguson J. J., 1982, Methods and principles of mycorrhizal research., P47

[10]

GERDEMANN J. W., 1963, TRANS BRIT MYCOL SOC, V46, P235

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