POLYXANTHYLIC ACID - STRUCTURES OF THE ORDERED FORMS

被引:24
作者
ROY, KB
FRAZIER, J
MILES, HT
机构
[1] Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Maryland, 20205, Bethesda
关键词
D O I
10.1002/bip.1979.360181213
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We present evidence for structures of two ordered forms of polyxanthylic acid based on ir spectroscopy, pH titrations, and thermal transitions. Over the pH range ∼6–9.5, the structure is a four‐stranded helix with alkali metal ions specifically complexed in the central channel. These internal counterions stabilize the structure by complexing with carbonyl oxygens and by partial screening of electrostatic repulsion caused by ionization of the xanthine residues in this pH range. Below pH 5, the structure is quite different and much more stable. Our data are consistent with a six‐stranded helix in which both carbonyl oxygens and both NH protons are hydrogen bonded. Copyright © 1979 John Wiley & Sons, Inc.
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页码:3077 / 3087
页数:11
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