NUCLEOSIDES AND NUCLEOTIDES .105. DNA BENDING IN D(A)(4)-D(T)(4) TRACTS CONTAINING 3-DEAZAADENINE OR 7-DEAZAADENINE SUBSTITUTED FOR ADENINE

被引：11

作者：

ONO, A ^{[1
]}

OHDOI, C ^{[1
]}

MATSUDA, A ^{[1
]}

UEDA, T ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA 12,NISHI 6,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1992年 / 11卷 / 2-4期

关键词：

D O I：

10.1080/07328319208021698

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Self-complementary decadeoxyribonucleotides containing 3-deazaadenine or 7-deazaadenine in place of one of the adenine moieties in a parent sequence, d(GAAAATTTTC)n, have been synthesized. After phosphorylation at their 5'-ends, the decamers were ligated to form multimers, which were analyzed by polyacrylamide gel electrophoresis. The multimers of the decamer containing 3-deazaadenine (3), d(GAA3ATTTTC)n, showed decreased bending compared with the multimers of d(GAAAATTTTC)n, while the decamer containing 3-deazaadenine at a different position, d(GAAA3TTTTC)n, did not show any degree of bending. Also, the properties of migration of the multimers containing 7-deazaadenine in the gel will be discussed.

引用

页码：227 / 235

页数：9

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