DETERMINATION OF PROLIFERATION INDEX WITH MIB-1 IN ADVANCED OVARIAN-CANCER USING QUANTITATIVE IMAGE-ANALYSIS

被引:59
作者
KERNS, BJM
JORDAN, PA
FAERMAN, LL
BERCHUCK, A
BAST, RC
LAYFIELD, LJ
机构
[1] DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710
[2] DUKE UNIV,MED CTR,DEPT IMMUNOL,DURHAM,NC
[3] DUKE UNIV,MED CTR,DEPT OBSTET & GYNECOL,DIV GYNECOL ONCOL,DURHAM,NC
[4] DUKE UNIV,MED CTR,DUKE COMPREHENS CANC CTR,DURHAM,NC 27710
[5] AMAC INC,WESTBROOK,ME
关键词
IMAGE CYTOMETRY; IMMUNOHISTOCHEMISTRY; MIB-1; OVARIAN CANCER;
D O I
10.1093/ajcp/101.2.192
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
A new monoclonal antibody MIB-1,reacts with the same epitope recognized by Ki-67. The authors investigated the feasibility of using image analysis to quantitate the MIB-1 staining (proliferation index [PI]) of epithelial ovarian cancers. The PI was determined in 50 advanced-stage primary ovarian cancers. Paraffin sections were immunostained with the MIB-1 monoclonal antibody, and the PI was calculated using a CAS 200(TM) image analyzer. Among 36 stage III ovarian carcinomas, the median PI was 15.19, compared with 18.9% in 14 stage IV cancers (P= .47). Based on exploratory methods, a cutoff point of 7% best dichotomized these patients into two prognostic groups. Of 39 patients whose cancers had a high MIB-1 expression (greater than or equal to 7%), the median survival was 16.5 months, which differed significantly (P=.01) from the median survival of 33.2 months observed in the 11 patients whose tumors demonstrated low MIB-1 expression (< 7%). Using MIB-1 as a binary variable, a strong correlation was found between overespression of c-erbB-2 (2(+) and 3(+)) and MIB-1 greater than or equal to 7% (P=.001). No relationship was found between PI and histologic grade. Further studies are warranted to investigate the relationship between MIB-1, PI expression, and other known clinicopathologic and genetic features of early- and late-stage ovarian cancer.
引用
收藏
页码:192 / 197
页数:6
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