SENSITIVE FLUORIMETRIC METHOD FOR DETERMINATION OF PLATELET-BOUND AND PLASMA-FREE SEROTONIN

被引：51

作者：

FRATTINI, P

CUCCHI, ML

SANTAGOSTINO, G

CORONA, GL

机构：

[1] Institute of Pharmacology, University of Pavia, Viale Taramelli 14

来源：

CLINICA CHIMICA ACTA | 1979年 / 92卷 / 03期

关键词：

D O I：

10.1016/0009-8981(79)90213-4

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A sensitive and specific fluorimetric assay for blood platelet-bound and plasma free serotonin is described. Serotonin is concentrated and purified on Amberlite CG-50 resin, eluted by 0.15 mol/1 HC1 and its native fluorescence is read at 295/350 nm. The good recovery (90% about), reproducibility and sensitivity (the smallest amount detectable is 5 ng) make the assay particularly useful for the detection of free serotonin in plasma. The whole procedure is rapid enough to permit the analysis of 15-20 samples within one working day and thus the method is suitable for routine determination of serotonin in biological specimens. © 1979.

引用

页码：353 / 360

页数：8

共 15 条

[1] AN IMPROVED METHOD FOR FLUORIMETRIC DETERMINATION OF 5-HYDROXYTRYPTAMINE IN TISSUES [J].

ANDEN, NE ;

MAGNUSSON, T .

ACTA PHYSIOLOGICA SCANDINAVICA, 1967, 69 (1-2) :87-+

[2] TOTAL 5-HYDROXYINDOLES IN BLOOD RELATED TO MIGRAINE ATTACKS [J].

BERSTAD, JR .

ACTA NEUROLOGICA SCANDINAVICA, 1976, 54 (04) :293-300

[3]

BLISS EL, 1973, SEROTONIN BEHAVIOR, P315

[4]

BOGGAN WO, 1973, SEROTONIN BEHAVIOR, P167

[5] BIOCHEMICAL BASIS OF MIGRAINE - CRITIQUE [J].

BRUYN, GW .

CLINICAL NEUROPHARMACOLOGY, 1976, 1 :185-213

[6]

COHEN DJ, 1974, ARCH GEN PSYCHIAT, V31, P845

[7] ESTIMATION OF 5-HYDROXYTRYPTAMINE IN HUMAN BLOOD [J].

CONTRACTOR, SF .

BIOCHEMICAL PHARMACOLOGY, 1964, 13 (09) :1351-&

[8]

CORONA GL, 1977, FARM ED PRAT, V32, P53

[9] PLASMA FREE SEROTONIN (5 HYDROXYTRYPTAMINE) [J].

CRAWFORD, N .

CLINICA CHIMICA ACTA, 1963, 8 (01) :39-&

[10]

DOUAY O, 1976, ANN BIOL CLIN-PARIS, V34, P47

← 1 2 →