SENSITIVE RADIOIMMUNOASSAY FOR SOMATOSTATIN USING N-I-125-TYR-SOMATOSTATIN AS LABELED ANTIGEN

被引：11

作者：

DUPONT, A

COY, DH

ALVARADOURBINA, G

COTE, J

MEYERS, CA

MCMANUS, J

BARDEN, N

DELEAN, A

LABRIE, F

机构：

[1] CTR HOSP UNIV LAVAL,SERV GASTROENTEROL,QUEBEC CITY G1V 4G2,QUEBEC,CANADA

[2] VET ADM HOSP,ENDOCRINE LAB,NEW ORLEANS,LA 70140

[3] VET ADM HOSP,POLYPEPTIDE LAB,NEW ORLEANS,LA 70140

来源：

CLINICAL ENDOCRINOLOGY | 1979年 / 10卷 / 01期

关键词：

D O I：

10.1111/j.1365-2265.1979.tb03032.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A sensitive radioimmunoassay for somatostatin using N‐[25I]‐Tyr‐somatostatin is described and compared with that using [125I]‐Tyr1‐somatostatin. The minimum detectable amount of somatostatin using N‐[1251]‐Tyr‐somatostatin as tracer was 0.1 to 0.5 pg, which is approximately 10‐fold lower than the lower detection limit of the RIA using [125I]‐Tyr1‐somatostatin. Moreover, it was found that the shelf‐life of N‐[1251]Tyr‐somatostatin was prolonged in comparison with labelled Tyr1‐somatostatin. Human pancreatic and gastric extracts displayed immunological similarity to synthetic somatostatin tetradecapeptide. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：47 / 54

页数：8

共 15 条

[1] SOMATOSTATIN - ABUNDANCE OF IMMUNOREACTIVE HORMONE IN RAT STOMACH AND PANCREAS [J].