CHROMATOGRAPHIC PURIFICATION AND CHARACTERIZATION OF EBV DNASE FROM CHEMICALLY-INDUCED LYMPHOID-CELLS

被引：7

作者：

HWANG, GY

LI, JKK

CHIOU, JF

TAN, RS

机构：

[1] CHINESE ACAD MED SCI,INST CANC,DEPT BIOCHEM,BEIJING,PEOPLES R CHINA

[2] NATL TAIWAN UNIV,COLL MED,DEPT BACTERIOL,TAIPEI,TAIWAN

来源：

VIROLOGY | 1990年 / 175卷 / 01期

关键词：

D O I：

10.1016/0042-6822(90)90214-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Epstein-Barr virus-associated deoxyribonuclease (EBV-DNase) was purified to homogeneity, as determined by silver staining, sequential column chromatography, and FPLC from Raji and P3HR-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate. This viral protein was immunogenic and elicited high neutralization titer sera in rabbits. By silver staining of SIDS-PAGE, Western immunoblot, and radioimmunoprecipitation using NPC patient sera and both polyclonal and monoclonal antibodies, the EBV DNase was identified as a 58K protein. The potential presence of two EBV DNases was also discussed. © 1990.

引用

页码：313 / 318

页数：6

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