PCR-GENERATED CONSPECIFIC SODIUM-CHANNEL GENE PROBE FOR THE HOUSE-FLY

被引：12

作者：

KNIPPLE, DC

PAYNE, LL

SODERLUND, DM

机构：

[1] Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 1991年 / 16卷 / 01期

关键词：

DNA AMPLIFICATION; VOLTAGE-SENSITIVE; PYRETHROID TARGET SITE;

D O I：

10.1002/arch.940160106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A segment of the house fly (Musca domestica) homologue of the para (paralytic) sodium channel gene of Drosophila melanogaster was isolated by using mixed sequence oligonucleotide primers in the polymerase chain reaction (PCR). The specificity of the procedure was demonstrated by genomic Southern analysis using the housefly PCR amplification product as a probe and by DNA sequence analysis. The latter showed structural homology to the para gene, but not to the corresponding region of DSC1, another D. melanogaster gene with structural similarity to vertebrate sodium channel genes.

引用

页码：45 / 53

页数：9

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