FREE TESTOSTERONE LEVELS IN PLASMA AND SALIVA AS DETERMINED BY A DIRECT SOLID-PHASE RADIOIMMUNOASSAY - A CRITICAL-EVALUATION

被引：21

作者：

REY, F

CHIODONI, G

BRAILLARD, K

BERTHOD, C

LEMARCHANDBERAUD, T

机构：

[1] Division d'Endocrinologie et Biochimie Clinique, Centre Hospitalier Universitaire Vaudois

来源：

CLINICA CHIMICA ACTA | 1990年 / 191卷 / 1-2期

关键词：

Free hormone radioimmunoassay; Free testosterone; Saliva testosterone;

D O I：

10.1016/0009-8981(90)90054-V

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We compared unbound (free) testosterone radioimmunoassay concentrations in plasma and saliva from men, using a direct radioimmunoassay kit involving a ligand analog of testosterone as tracer. The assay failed to reveal detectable testosterone concentrations in saliva. In plasma the free testosterone levels were about 4 times lower than those obtained by calculation or ultrafiltration methods. Moreover, unexpected similar free testosterone levels were obtained in samples comparable in their total testosterone content but distinct in their steroid binding protein content (buffered testosterone dilutions). We suspect that free testosterone levels determined with this direct radioimmunoassay probably do not reflect the true free testosterone values and conclude that their significance remains to be established. © 1990.

引用

页码：21 / 30

页数：10

共 17 条

[1] SALIVARY TESTOSTERONE - RELATIONSHIP TO UN-BOUND PLASMA TESTOSTERONE IN NORMAL AND HYPERANDROGENIC WOMEN [J].