EXTRACELLULAR PROTEASE AND AMYLASE ACTIVITIES IN LIGNINASE-PRODUCING LIQUID CULTURE OF PHANEROCHAETE-CHRYSOSPORIUM

被引：15

作者：

DEY, S

MAITI, TK

SAHA, N

BANERJEE, R

BHATTACHARYYA, BC

机构：

[1] Biotechnology Unit, Department of Chemical Engineering, Indian Institute of Technology, Kharagpur

来源：

PROCESS BIOCHEMISTRY | 1991年 / 26卷 / 06期

关键词：

D O I：

10.1016/0032-9592(91)85021-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of extracellular proteases and alpha-amylase by ligninase-producing liquid culture of the white-rot fungus Phanerochaete chrysosporium NCIM 1197 has been investigated in stationary culture conditions. Acid, neutral, and alkaline proteases were all identified with maximum activities on the 9th day of incubation. Peak production of ligninase and alpha-amylase occurred on day 6 and day 3 respectively. The time courses of the production of proteases and those of ligninase and amylase were negatively correlated. Protease inhibition by the addition of phenylmethyl sulphonyl fluoride (days 1 and 4) resulted in enhanced activities of ligninase and amylase beyond the 9th day, suggesting an effect of protease on ligninase and amylase activity.

引用

页码：325 / 329

页数：5

共 21 条

[1]

BANERJEE R, 1990, THESIS IIT KHARAGPUR

[2] AMYLASES, ALPHA AND BETA [J].