ON THE TOXICITY OF LOW-DOSES OF TETRASODIUM-ETHYLENEDIAMINE-TETRAACETATE (NA-EDTA) IN NORMAL RAT-KIDNEY (NRK) CELLS IN CULTURE

被引：19

作者：

HUGENSCHMIDT, S ^{[1
]}

PLANASBOHNE, F ^{[1
]}

TAYLOR, DM ^{[1
]}

机构：

[1] KERNFORSCHUNGSZENTRUM KARLSRUHE GMBH,INST GENET & TOXIKOL,POSTFACH 3640,W-7500 KARLSRUHE 1,GERMANY

来源：

ARCHIVES OF TOXICOLOGY | 1993年 / 67卷 / 01期

关键词：

EDTA; CYTOTOXICITY; KIDNEY CELL CULTURES;

D O I：

10.1007/BF02072040

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The toxicity of tetrasodium-ethylenediaminetetraacetate (Na-EDTA) was investigated in normal rat kidney cells (NRK-52E, American Type Culture Collection) in culture. Cell death and reduced colony-forming ability were observed at Na-EDTA concentrations < 100 muM, that is at concentrations lower than that required to chelate all the Ca2+ in the growth medium. No toxicity was observed when cells were exposed to 100 muM Zn- or Fe-EDTA, but the same concentration of Cu-EDTA was as toxic as Na-EDTA. Continuous exposure of the cell cultures to 5 muM Na- or Zn-EDTA for up to 7 weeks yielded no indications of toxicity.

引用

页码：76 / 78

页数：3

共 7 条

[1]

BOHNE F, 1972, STRAHLENTHER ONKOL, V143, P106

[2]

Brauch H., 1987, VOM WASSER, V69, P155

[3] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].