AN IMPROVED RT-PCR PROTOCOL FOR THE QUANTITATION OF HUMAN RETINOIC ACID RECEPTOR RNA

被引：10

作者：

FERRARI, N ^{[1
]}

PFEFFER, U ^{[1
]}

TOSETTI, F ^{[1
]}

BRIGATI, C ^{[1
]}

VIDALI, G ^{[1
]}

机构：

[1] IST NAZL RIC CANC,MOLEC BIOL LAB,I-16132 GENOA,ITALY

来源：

EXPERIMENTAL CELL RESEARCH | 1994年 / 211卷 / 01期

关键词：

D O I：

10.1006/excr.1994.1067

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) system has been developed to calculate the level of expression of human retinoic acid receptors (hRAR) alpha, beta, and gamma. Starting from a single cDNA preparation, the system allows the measurement of the number of molecules of each mRNA receptor. This is made possible by a synthetic internal standard mRNA which is added in known concentrations at the beginning of the reaction. The system is tested in a rhabdomyosarcoma cell line (A-673) where we have measured the upregulation of beta and gamma receptor mRNAs following treatment with retinoic acid. (C) 1994 Academic Press, Inc.

引用

页码：121 / 126

页数：6

共 8 条

[1] A NEW RETINOIC ACID RECEPTOR IDENTIFIED FROM A HEPATOCELLULAR-CARCINOMA [J].