HYDROXYNITRILE LYASE FROM SORGHUM-BICOLOR - A GLYCOPROTEIN HETEROTETRAMER

被引:45
作者
WAJANT, H
MUNDRY, KW
机构
[1] Biological Institute, Department of Botany, Stuttgart University, 7000 Stuttgart 80
关键词
HYDROXYNITRILE LYASE; SORGHUM-BICOLOR; CYANOGENESIS;
D O I
10.1016/0168-9452(93)90120-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Highly purified hydroxynitrile lyase from Sorghum bicolor L. (EC 4.1.2.11) is composed of two different subunits, alpha and beta. The structure of the active enzyme is 2alpha2beta. It was shown in an immunoblot assay that both subunits are glycoproteins. The nature of the carbohydrate residues was further investigated by means of biotin-labeled lectins. The influence of glycosylation on the mobility of hydroxynitrile lyase in SDS-PAGE was demonstrated with enzymatically deglycosylated protein. Enzyme assays with hydroxynitrile lyase, which was partially deglycosylated at reversible denaturing conditions, suggested that the sugar residues are necessary for the protein to maintain its functional structure.
引用
收藏
页码:127 / 133
页数:7
相关论文
共 17 条
[1]   LECTINS AS PROBES FOR THE ASSAY OF RHABDOVIRUS INFECTIONS IN PLANTS [J].
ADAM, G ;
HEEGARD, P ;
BOGHANSEN, TC ;
MUNDRY, KW .
JOURNAL OF VIROLOGICAL METHODS, 1987, 17 (3-4) :263-275
[2]  
BECKER W, 1963, BIOCHEM Z, V337, P156
[3]  
BOVE C, 1961, J BIOL CHEM, V236, P207
[4]  
CARVALHO FJP, 1981, THESIS U CALIFORNIA
[5]  
Conn E.E., 1981, BIOCH PLANTS COMPREH, P479, DOI 10.1016/B978-0-12-675407-0.50022-1
[6]  
GARSTNER E, 1975, HOPESEYLERS Z PHYSL, V356, P1853
[7]   FLAVOENZYME HYDROXYNITRIL LYASE (D-OXYNITRILASE) [J].
GERSTNER, E ;
PFEIL, E .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1972, 353 (03) :271-&
[8]   CHEMICAL AND BIOLOGICAL CLASSIFICATION OF ROSACEAE . D-HYDROXYNITRILASE A FLAVIN SYSTEM [J].
GERSTNER, E ;
MATZKE, V ;
PFEIL, E .
NATURWISSENSCHAFTEN, 1968, 55 (12) :561-&
[9]   GLUCOSIDASES SPECIFIC FOR CYANOGENIC GLUCOSIDE TRIGLOCHININ - PURIFICATION AND CHARACTERIZATION OF BETA-GLUCOSIDASES FROM ALOCASIA MACRORRHIZA SCHOTT [J].
HOSEL, W ;
NAHRSTEDT, A .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1975, 356 (08) :1265-1275
[10]   MANDELONITRILE LYASE FROM XIMENIA-AMERICANA L - STEREOSPECIFICITY AND LACK OF FLAVIN PROSTHETIC GROUP [J].
KUROKI, GW ;
CONN, EE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :6978-6981