P80-FORM OF THE HUMAN TUMOR-NECROSIS-FACTOR RECEPTOR IS INVOLVED IN DNA FRAGMENTATION

被引：28

作者：

HIGUCHI, M ^{[1
]}

AGGARWAL, BB ^{[1
]}

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CLIN IMMUNOL & BIOL THERAPY,HOUSTON,TX 77030

来源：

FEBS LETTERS | 1993年 / 331卷 / 03期

关键词：

TUMOR NECROSIS FACTOR; LYMPHOTOXIN; APOPTOSIS; TNF RECEPTOR; DNA FRAGMENTATION; DOWN-MODULATION;

D O I：

10.1016/0014-5793(93)80347-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two different types of TNF receptors with molecular masses of 60 kDa (p60) and 80 kDa (p80) have been identified. TNF is known to cause DNA fragmentation in certain tumor cell lines but the role of p60 and p80 in this action is not understood. In the present study, we examined the role of these receptors in TNF-induced DNA fragmentation. Treatment of U-937 cells with phorbol ester caused downregulation of both types of TNF receptors and this was accompanied by disappearance of the TNF-induced DNA fragmentation. The removal of phorbol ester led to two time-dependent events: (1) the rapid regeneration of the p80 form but not the p60 form of the TNF receptor; and (2) the reappearance of TNF-induced DNA fragmentation. These results suggest that the p80 receptor could mediate the TNF-induced DNA fragmentation.

引用

页码：252 / 255

页数：4

共 25 条

[1]

AGGARWAL BB, 1993, LYMPHOKINE CYTOK RES, V12, P149

[2] CHARACTERIZATION OF RECEPTORS FOR HUMAN-TUMOR NECROSIS FACTOR AND THEIR REGULATION BY GAMMA-INTERFERON [J].

AGGARWAL, BB ;

EESSALU, TE ;

HASS, PE .

NATURE, 1985, 318 (6047) :665-667

[3]

AGGARWAL BB, 1987, J BIOL CHEM, V262, P16450

[4] IDENTIFICATION OF 2 TYPES OF TUMOR-NECROSIS-FACTOR RECEPTORS ON HUMAN CELL-LINES BY MONOCLONAL-ANTIBODIES [J].

BROCKHAUS, M ;

SCHOENFELD, HJ ;

SCHLAEGER, EJ ;

HUNZIKER, W ;

LESSLAUER, W ;

LOETSCHER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3127-3131

[5] ENDOTOXIN-INDUCED SERUM FACTOR THAT CAUSES NECROSIS OF TUMORS [J].

CARSWELL, EA ;

OLD, LJ ;

KASSEL, RL ;

GREEN, S ;

FIORE, N ;

WILLIAMSON, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3666-3670

[6]

ELIAS L, 1991, LEUKEMIA, V5, P879

[7]

ENGLEMANN H, 1990, J BIOL CHEM, V265, P14497

[8] INDEPENDENT REGULATION OF 55-KDA AND 75-KDA TUMOR-NECROSIS-FACTOR RECEPTORS DURING ACTIVATION OF HUMAN PERIPHERAL-BLOOD LYMPHOCYTES-B [J].

ERIKSTEIN, BK ;

SMELAND, EB ;

BLOMHOFF, HK ;

FUNDERUD, S ;

PRYDZ, K ;

LESSLAUER, W ;

ESPEVIK, T .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (04) :1033-1037

[9] CHARACTERIZATION OF BINDING AND BIOLOGICAL EFFECTS OF MONOCLONAL-ANTIBODIES AGAINST A HUMAN TUMOR NECROSIS FACTOR RECEPTOR [J].

ESPEVIK, T ;

BROCKHAUS, M ;

LOETSCHER, H ;

NONSTAD, U ;

SHALABY, R .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (02) :415-426

[10]

GREENBLATT MS, 1992, BLOOD, V80, P1339

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