THE CDNA STRUCTURE OF THE PORCINE PRO-HORMONE CONVERTASE PC2 AND THE COMPARATIVE PROCESSING BY PC1 AND PC2 OF THE N-TERMINAL GLYCOPEPTIDE SEGMENT OF PORCINE POMC

被引：36

作者：

SEIDAH, NG

FOURNIER, H

BOILEAU, G

BENJANNET, S

RONDEAU, N

CHRETIEN, M

机构：

[1] CLIN RES INST MONTREAL,MOLEC NEUROENDOCRINOL LAB,MONTREAL H2W 1R7,QUEBEC,CANADA

[2] UNIV MONTREAL,DEPT BIOCHEM,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

FEBS LETTERS | 1992年 / 310卷 / 03期

关键词：

PORCINE PC2; PRO-HORMONE CONVERTASE; VACCINIA VIRUS EXPRESSION; POMC N-TERMINAL GLYCOPEPTIDE CLEAVAGE;

D O I：

10.1016/0014-5793(92)81339-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete cDNA structure of the porcine (p) pro-protein and pro-hormone convertase PC2 (pPC2) was obtained from a cDNA library of pituitary neurointermediate lobes mRNA. The deduced amino acid sequence revealed that pPC2 exhibits a 99-97% sequence identity to the human, mouse and rat homologues. The 3' end of the 2.1 kb cDNA is the least conserved segment. On Northern blots of pars intermedia poly A+ RNA two transcripts of 3 and 5 kb were detected. Molecular analysis of the N-terminal glycopeptide products of porcine pro-opiomelanocortin (pPOMC) co-expressed with vaccinia virus recombinants of PC1 or PC2. revealed that in cells devoid or containing secretory granules both convertases can cleave pPOMC with PC1 releasing the 1-80, 1-107 and 1 148 glycopeptide fragments, and PC2 cleaving pPOMC directly into pPOMC 1-107.

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页码：235 / 239

页数：5

相关论文

共 25 条

[1] PC1 AND PC2 ARE PROPROTEIN CONVERTASES CAPABLE OF CLEAVING PROOPIOMELANOCORTIN AT DISTINCT PAIRS OF BASIC RESIDUES [J].

BENJANNET, S ;

RONDEAU, N ;

DAY, R ;

CHRETIEN, M ;

SEIDAH, NG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3564-3568

[2]

BENJANNET S, 1992, J BIOL CHEM, V267, P11417

[3] PROHORMONE-CONVERTING ENZYMES - REGULATION AND EVALUATION OF FUNCTION USING ANTISENSE RNA [J].

BLOOMQUIST, BT ;

EIPPER, BA ;

MAINS, RE .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (12) :2014-2024

[4] COMPLETE STRUCTURE OF THE PORCINE PRO-OPIOMELANOCORTIN MESSENGER-RNA DERIVED FROM THE NUCLEOTIDE-SEQUENCE OF CLONED CDNA [J].

BOILEAU, G ;

BARBEAU, C ;

JEANNOTTE, L ;

CHRETIEN, M ;

DROUIN, J .

NUCLEIC ACIDS RESEARCH, 1983, 11 (22) :8063-8071

[5] CHARACTERIZATION OF MULTIPLE FORMS OF PORCINE ANTERIOR-PITUITARY PROOPIOMELANOCORTIN AMINO-TERMINAL GLYCOPEPTIDE [J].

BOILEAU, G ;

LARIVIERE, N ;

HSI, KL ;

SEIDAH, NG ;

CHRETIEN, M .

BIOCHEMISTRY, 1982, 21 (21) :5341-5346

[6] STRUCTURE AND EXPRESSION OF XENOPUS PROHORMONE CONVERTASE PC2 [J].

BRAKS, JAM ;

GULDEMOND, KCW ;

VANRIEL, MCHM ;

COENEN, AJM ;

MARTENS, GJM .

FEBS LETTERS, 1992, 305 (01) :45-50

[7] SITE-DIRECTED MUTAGENESIS AND THE ROLE OF THE OXYANION HOLE IN SUBTILISIN [J].

BRYAN, P ;

PANTOLIANO, MW ;

QUILL, SG ;

HSIAO, HY ;

POULOS, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3743-3745

[8] PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST THE N-TERMINAL GLYCOPEPTIDE OF PORCINE PROOPIOMELANOCORTIN - THEIR USE FOR SOLID-PHASE RADIOIMMUNOASSAY, WESTERN BLOTTING, AND IMMUNOGOLD CYTOCHEMISTRY [J].

CHEVRIER, D ;

LEMIEUX, E ;

FYFE, M ;

LABONTE, N ;

ZOLLINGER, M ;

BENNETT, HPJ ;

CRINE, P .

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1991, 69 (01) :58-65

[9] NEW VACCINIA VIRUS RECOMBINATION PLASMIDS INCORPORATING A SYNTHETIC LATE PROMOTER FOR HIGH-LEVEL EXPRESSION OF FOREIGN PROTEINS [J].

DAVISON, AJ ;

MOSS, B .

NUCLEIC ACIDS RESEARCH, 1990, 18 (14) :4285-4286

[10] DISTRIBUTION AND REGULATION OF THE PROHORMONE CONVERTASES PC1 AND PC2 IN THE RAT PITUITARY [J].

DAY, R ;

SCHAFER, MKH ;

WATSON, SJ ;

CHRETIEN, M ;

SEIDAH, NG .

MOLECULAR ENDOCRINOLOGY, 1992, 6 (03) :485-497