CLONING AND EXPRESSION OF RAT PROSTAGLANDIN ENDOPEROXIDE SYNTHASE (CYCLOOXYGENASE)-2 CDNA

被引：117

作者：

KENNEDY, BP ^{[1
]}

CHAN, CC ^{[1
]}

CULP, SA ^{[1
]}

CROMLISH, WA ^{[1
]}

机构：

[1] MERCK FROSST CANADA INC, MERCK FROSST CTR THERAPEUT RES, DEPT PHARMACOL, DORVAL H9R 4P8, PQ, CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat prostaglandin endoperoxidase synthase-2 (PGHS-2) cDNA was cloned from rat calvarial osteoblasts total RNA by RT-PCR. The primary sequence of rat PGHS-2 had 98% and 92% identity to the mouse and human enzymes, respectively. Transfection of the rat PGHS-2 cDNA into COS 7 cells, followed by the addition of 20 μM arachidonic acid, resulted in a dramatic increase in PGE2 released from these cells. The amount of PGE2 produced was comparable to that obtained from cells similarly transfected with human PGHS-1 cDNA. In the rat paw carrageenin-oedema inflammatory model, the injected paw had elevated levels of PGHS-2 mRNA compared to the control paw. In a rat pyrexia model, injection of the pyrogen lipopolysaccharide, resulted in elevated levels of PGHS-2 mRNA in the brain. These results suggest that PGHS-2 expression plays a role both in inflammation and fever. © 1993 Academic Press, Inc.

引用

页码：494 / 500

页数：7

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