U20, A NOVEL SMALL NUCLEOLAR RNA, IS ENCODED IN AN INTRON OF THE NUCLEOLIN GENE IN MAMMALS

被引:52
作者
NICOLOSO, M [1 ]
CAIZERGUESFERRER, M [1 ]
MICHOT, B [1 ]
AZUM, MC [1 ]
BACHELLERIE, JP [1 ]
机构
[1] UNIV TOULOUSE 3,CNRS,BIOL MOLEC EUCARYOTE LAB,F-31062 TOULOUSE,FRANCE
关键词
D O I
10.1128/MCB.14.9.5766
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have found that intron 11 of the nucleolin gene in humans and rodents encodes a previously unidentified small nucleolar RNA, termed U20. The single-copy U20 sequence is located on the same DNA strand as the nucleolin mRNA. U20 RNA, which does not possess a trimethyl cap, appears to result from intronic RNA processing and not from transcription of an independent gene. In mammals, U20 RNA is an 80-nucleotide-long, metabolically stable species, present at about 7 x 10(3) molecules per exponentially growing HeLa cell. It has a nucleolar localization, as indicated by fluorescence microscopy following in situ hybridization with digoxigenin-labeled oligonucleotides. U20 RNA contains the box C and hox D sequence motifs, hallmarks of most small nucleolar RNAs reported to date, and is immunoprecipitated by antifibrillarin antibodies. It also exhibits a 5'-3' terminal stem bracketing the box C-bos D motifs like U14, U15, U16! or Y RNA, A U20 homolog of similar size has been detected in all vertebrate classes by Northern (RNA) hybridization,vith mammalian oligonucleotide probes. U20 RNA contains an extended region (21 nucleotides) of perfect complementarity with a phylogenetically conserved sequence in 188 rRNA. This complementarity is strongly preserved among distant vertebrates, suggesting that U20 RNA may be involved in the formation of the small ribosomal subunit like nucleolin, the product of its host gene.
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页码:5766 / 5776
页数:11
相关论文
共 69 条
[1]   CHANGES IN RNA IN RELATION TO GROWTH OF FIBROBLAST .2. LIFETIME OF MESSENGER-RNA, RIBOSOMAL-RNA, AND TRANSFER-RNA IN RESTING AND GROWING CELLS [J].
ABELSON, HT ;
JOHNSON, LF ;
PENMAN, S ;
GREEN, H .
CELL, 1974, 1 (04) :161-165
[2]   DIRECT RIBOSOMAL-RNA SEQUENCING FOR PHYLOGENETIC STUDIES [J].
BACHELLERIE, JP ;
QU, LH .
MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, 1993, 224 :349-357
[3]   ULTRASTRUCTURAL ORGANIZATION AND BIOCHEMICAL CHARACTERIZATION OF CHROMATIN-RNA-PROTEIN COMPLEXES ISOLATED FROM MAMMALIAN-CELL NUCLEI [J].
BACHELLERIE, JP ;
PUVION, E ;
ZALTA, JP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 58 (02) :327-337
[4]   SNR31, SNR32, AND SNR33 - 3 NOVEL, NONESSENTIAL SNRNAS FROM SACCHAROMYCES-CEREVISIAE [J].
BALAKIN, AG ;
SCHNEIDER, GS ;
CORBETT, MS ;
NI, JW ;
FOURNIER, MJ .
NUCLEIC ACIDS RESEARCH, 1993, 21 (23) :5391-5397
[5]   SNR30 - A NEW, ESSENTIAL SMALL NUCLEAR-RNA FROM SACCHAROMYCES-CEREVISIAE [J].
BALLY, M ;
HUGHES, J ;
CESARENI, G .
NUCLEIC ACIDS RESEARCH, 1988, 16 (12) :5291-5303
[6]   AN INTACT BOX-C SEQUENCE IN THE U3 SNRNA IS REQUIRED FOR BINDING OF FIBRILLARIN, THE PROTEIN COMMON TO THE MAJOR FAMILY OF NUCLEOLAR SNRNPS [J].
BASERGA, SJ ;
YANG, XDW ;
STEITZ, JA .
EMBO JOURNAL, 1991, 10 (09) :2645-2651
[7]   IDENTIFICATION AND FUNCTIONAL-ANALYSIS OF 2 U3 BINDING-SITES ON YEAST PRERIBOSOMAL RNA [J].
BELTRAME, M ;
TOLLERVEY, D .
EMBO JOURNAL, 1992, 11 (04) :1531-1542
[8]   NUCLEOLAR LOCALIZATION OF MYC TRANSCRIPTS [J].
BOND, VC ;
WOLD, B .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (06) :3221-3230
[9]   NUCLEOLIN GENE ORGANIZATION IN RODENTS - HIGHLY CONSERVED SEQUENCES WITHIN 3 OF THE 13 INTRONS [J].
BOURBON, HM ;
AMALRIC, F .
GENE, 1990, 88 (02) :187-196
[10]  
CAIZERGUESFERRER M, 1991, DEVELOPMENT, V112, P317