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CHEMILUMINESCENCE METHOD FOR THE DETERMINATION OF ADENINE AFTER REACTION WITH PHENYLGLYOXAL

被引：20
作者：
KURODA, N
NAKASHIMA, K
AKIYAMA, S
机构：
[1] School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, 852, Bunkyo-machi
来源：
ANALYTICA CHIMICA ACTA | 1993年 / 278卷 / 02期
关键词：
CHEMILUMINESCENCE; ADENINE; ADENOSINE; NUCLEIC ACID BASES;
D O I：
10.1016/0003-2670(93)85110-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A chemiluminescence (CL) method is described for the determination of adenine. Treatment of adenine with phenylglyoxal in acidic propan-2-ol gave products that produced CL in the presence of hydrogen peroxide, 2-mercaptoethanol and sodium hydroxide. The proposed method is highly sensitive and selective to adenine and adenosine. Adenine can be determined in the range 1.5 x 10(-4)-5.0 x 10(-7) M. The detection limit for adenine is 1.9 x 10(-7) M (19 pmol per assay).
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页码：275 / 278
页数：4
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