INOSITOL TRISPHOSPHATE-MEDIATED CA-2+ RELEASE IN BEET MICROSOMES IS INHIBITED BY HEPARIN

被引：66

作者：

BROSNAN, JM

SANDERS, D

机构：

[1] Biology Department, University of York, York

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

(Beta vulgaris); Calcium efflux; Heparin; Inositol 1,4,5-trisphosphate; Tonoplast;

D O I：

10.1016/0014-5793(90)80068-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microsomes from the storage root of red beet exhibit ATP-dependent, protonophore-sensitive Ca2+ accumulation, characteristic of the vacuolar membrane (tonoplast). A portion (20%) of this intravesicular Ca2+ store is specifically released by inositol 1,4,5-trisphosphate (InsP3) with a K0.5 = 0.54 μM. InsP3,-mediated Ca2+ release is eliminated by low concentrations of heparin (K0.5 = 86 nM). This result highlights the conservation between the animal and plant inositol 1,4,5-trisphosphate-mediated Ca2+ release mechanisms, despite the fact that they are located at different intracellular membranes. © 1990.

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页码：70 / 72

页数：3

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