HIGH-PERFORMANCE ELECTROPHORESIS INCLUDING SEPARATION OF NUCLEIC-ACIDS AND THEIR DEGRADATION PRODUCTS - INTERPLAY BETWEEN THEORY AND PRACTICAL EXPERIMENTS

被引：6

作者：

HJERTEN, S

机构：

[1] Department of Biochemistry, University of Uppsala, Biomedical Center, Uppsala

来源：

NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 03期

关键词：

D O I：

10.1080/07328319008045143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The analogy between electrophoresis, centrifugation and chromatography is stressed by consideration of a formula which is valid for all three methods. An equation for electrophoretic plate heights forms the basis for a discussion of the experimental conditions for maximum resolution. The difference between true and apparent plate numbers and plate heights is underlined. Separations of nucleotides and DNA fragments are presented. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：319 / 330

页数：12

共 31 条

[1] ELECTROPHORETIC THERMAL THEORY .2. STEADY-STATE RADIAL TEMPERATURE-GRADIENTS IN CIRCULAR SECTION COLUMNS
BROWN, JF
HINCKLEY, JON
[J]. JOURNAL OF CHROMATOGRAPHY, 1975, 109 (02): : 218 - 224
[2] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD
CARLE, GF
FRANK, M
OLSON, MV
[J]. SCIENCE, 1986, 232 (4746) : 65 - 68
[3] COHEN A, 1987, ANAL CHEM, V59, P102
[4] RAPID SEPARATION AND PURIFICATION OF OLIGONUCLEOTIDES BY HIGH-PERFORMANCE CAPILLARY GEL-ELECTROPHORESIS
COHEN, AS
NAJARIAN, DR
PAULUS, A
GUTTMAN, A
SMITH, JA
KARGER, BL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9660 - 9663
[5] EFFICIENCY OF GEL-ELECTROPHORESIS
COX, HC
TEVEN, JMG
HESSELS, JKC
[J]. JOURNAL OF CHROMATOGRAPHY, 1972, 66 (01): : 19 - &
[6] CAPILLARY ZONE ELECTROPHORESIS - QUANTITATIVE STUDY OF THE EFFECTS OF SOME DISPERSIVE PROCESSES ON THE SEPARATION EFFICIENCY
FORET, F
DEML, M
BOCEK, P
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 452 : 601 - 613
[7] EFFECT OF TEMPERATURE-GRADIENTS ON THE EFFICIENCY OF CAPILLARY ZONE ELECTROPHORESIS SEPARATIONS
GRUSHKA, E
MCCORMICK, RM
KIRKLAND, JJ
[J]. ANALYTICAL CHEMISTRY, 1989, 61 (03) : 241 - 246
[8] HJALMARSSON SG, 1981, CRC CRIT R ANAL CHEM, P261
[9] Hjerten S, 1967, Chromatogr Rev, V9, P122, DOI 10.1016/0009-5907(67)80003-6
[10] HIGH-PERFORMANCE ELECTROPHORESIS - ELIMINATION OF ELECTROENDOSMOSIS AND SOLUTE ADSORPTION
HJERTEN, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 347 (02): : 191 - 198

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