AN AUTOCLAVABLE GLUCOSE BIOSENSOR FOR MICROBIAL FERMENTATION MONITORING AND CONTROL

被引：19

作者：

PHELPS, MR

HOBBS, JB

KILBURN, DG

TURNER, RFB

机构：

[1] UNIV BRITISH COLUMBIA,DEPT CHEM ENGN,VANCOUVER,BC V6T 1Z3,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT MICROBIOL & IMMUNOL,VANCOUVER,BC V6T 1Z3,CANADA

[3] UNIV BRITISH COLUMBIA,DEPT ELECT ENGN,VANCOUVER,BC V6T 1Z3,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 46卷 / 06期

关键词：

GLUCOSE BIOSENSOR; BIOSENSOR; GLUCOSE OXIDASE; CELLULOSE BINDING DOMAIN (CBD); FERMENTATION MONITORING;

D O I：

10.1002/bit.260460604

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The design, construction, and characterization of a prototype-regenerable glucose biosensor based on the reversible immobilization of glucose oxidase (GOx) using cellulose binding domain (CBD) technology is described. GOx, chemically linked to CBD, is immobilized by binding to a cellulose matrix on the sensor-indicating electrode. Enzyme immobilization can be reversed by perfusing the cellulose matrix with a suitable eluting solution. An autoclavable sensor membrane system is employed which is shown to be practical for use in real microbial fermentations. The prototype glucose biosensor was used without failure or deterioration during fed-batch fermentations of Escherichia coli reaching a maximum cell density of 85 g (dry weight)/L. Medium glucose concentration based on sensor output correlated closely with off-line glucose analysis and was controlled manually at 0.44 +/- 0.2 g/L for 2 h based on glucose sensor output. The sensor enzyme component could be eluted and replaced without interrupting the fermentation. To our knowledge, no other in situ biosensor has been used for such an extended period of time in such a high-cell-density fermentation. (C) 1995 John Wiley & Sons, Inc.

引用

页码：514 / 524

页数：11

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