MATRIX-ASSISTED LASER-DESORPTION IONIZATION OF OLIGONUCLEOTIDES WITH VARIOUS MATRICES

被引：55

作者：

TANG, K ^{[1
]}

ALLMAN, SL ^{[1
]}

CHEN, CH ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,POB 2008,BLDG 5500,MS-6378,OAK RIDGE,TN 37831

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 10期

关键词：

D O I：

10.1002/rcm.1290071016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ferulic acid, 3-hydroxypicolinic acid (3-HPA), and 2,5-dihydroxybenzoic acid (2,5-DHB) were used as matrices in MALDI of various oligonucleotides. It was found that 2,5-DHB is an excellent matrix for polydeoxyribothymidylic acid. A poly-T oligonucleotide with a size of 130 bases was successfully detected. 3-HPA was found to be a good matrix for MALDI of homo-oligomeric deoxynucleotides as well as those containing four different bases. Parent ions of pd(A)60, d(T)100, d(G)40, and d(C)40 were observed, and a mixed-base oligonucleotide of 150-mer was also detected. Polymer ions of poly-A as large as 420-mer were measured; however, the efficiency for detecting poly-T with 3-HPA as a matrix was slightly worse than when 2,5-DHB was used. Comparisons of three matrices for various oligonucleotides is also presented.

引用

页码：943 / 948

页数：6

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