PLATELET-ACTIVATING-FACTOR ACETYLHYDROLASE (PAF-AH) IN HUMAN KIDNEY

被引：27

作者：

ANTONOPOULOU, S

DEMOPOULOS, CA

IATROU, C

MOUSTAKAS, G

ZIROGIANNIS, P

机构：

[1] UNIV ATHENS,DEPT CHEM,GR-15771 ATHENS,GREECE

[2] GEN HOSP ATHENS,DEPT NEPHROL,GR-11527 ATHENS,GREECE

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 09期

关键词：

D O I：

10.1016/0020-711X(94)90138-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. PAF-AH activity in human kidney (cortex and medulla) has been demonstrated and shares the following properties. 2. Does not require the presence of Ca2+ and appears to be different from phospholipase A(2). 3. The pH optimum shows a peak at 7-7.4. 4. It is stable for 4 days at -30 degrees C. 5. It is mainly distributed in the microsomal fraction. 6. The apparent K-m, values of the enzymes of cortex and medulla are 0.553 and 0.207 mu M, respectively and distinct from serum PAF-AH (1.439 mu M). 7. The apparent molecular weight values are 60,000 and 25,000 for medulla and cortex, respectively and distinct from serum PAF-AH (94,000).

引用

页码：1157 / 1162

页数：6

共 11 条

[1]

BLANK ML, 1981, J BIOL CHEM, V256, P175

[2]

FARR RS, 1983, FED PROC, V42, P3120

[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[4]

LEE TC, 1985, PHOSPHOLIPIDS CELLUL, V2, P1

[5]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[6] CHARACTERIZATION OF SERUM PLATELET-ACTIVATING FACTOR (PAF) ACETYLHYDROLASE - CORRELATION BETWEEN DEFICIENCY OF SERUM PAF ACETYLHYDROLASE AND RESPIRATORY SYMPTOMS IN ASTHMATIC-CHILDREN [J].

MIWA, M ;

MIYAKE, T ;

YAMANAKA, T ;

SUGATANI, J ;

SUZUKI, Y ;

SAKATA, S ;

ARAKI, Y ;

MATSUMOTO, M .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (06) :1983-1991

[7]

PINCKARD RN, 1986, FASEB J, V45, P856

[8]

SNYDER F, 1987, METHOD ENZYMOL, V141, P379

[9]

STAFFORINI DM, 1987, J BIOL CHEM, V262, P4223

[10]

STREMLER KE, 1989, J BIOL CHEM, V264, P5331

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