CLONING OF HERPES-SIMPLEX TYPE-1 DNA FRAGMENTS IN A BACTERIOPHAGE-LAMBDA VECTOR

被引：81

作者：

ENQUIST, LW

MADDEN, MJ

SCHIOPSTANSLY, P

VANDEWOUDE, GF

机构：

[1] Laboratory of Molecular Virology, National Cancer Institute, Bethesda

来源：

SCIENCE | 1979年 / 203卷 / 4380期

关键词：

D O I：

10.1126/science.216076

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA isolated from defective and nondefective virions of herpes simplex type I (HSV-1) (strain Patton) was digested with restriction endonucleases, and the resulting DNA fragments were inserted in the EK2 coliphage vector λgtWES·λB. The recombinant DNA was encapsidated in vitro under P4 maximum containment conditions. These λ-HSV1 hybrids were purified and amplified, and the DNA was isolated in the P4 facility. DNA, free of viable phage and bacteria, was removed from P4 conditions and analyzed. Represented among the hybrids studied to date are DNA fragments from about 50 percent of the normal HSV-1 genome. The hybrids derived from defective HSV-1 DNA fragments demonstrate the existence of many similar but not identical classes of defective genomes. Copyright © 1979 AAAS.

引用

页码：541 / 544

页数：4

共 30 条

[1] HERPES SIMPLEX VIRUS DNA [J].