ACTION OF SPERMIDINE, N-1-ACETYLSPERMIDINE, AND N-8-ACETYLSPERMIDINE AT APURINIC SITES IN DNA

被引：7

作者：

HAUKANES, BI ^{[1
]}

SZAJKO, K ^{[1
]}

HELLAND, DE ^{[1
]}

机构：

[1] POLISH ACAD SCI,DEPT BIOGEN AMINES,PL-90362 LODZ 40,POLAND

来源：

FEBS LETTERS | 1990年 / 269卷 / 02期

关键词：

Apurinic/apyrimidinic (AP) site; DNA repair; Polyamine;

D O I：

10.1016/0014-5793(90)81200-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cleavage efficiency of spermidine and its acetyl derivatives (N1-acetylspermidine and N8-acetylspennidine) at apurinic sites in DNA were examined by PAGE-urea analysis. The three polyamines induced different rates of cleavage when compared at 1 mM concentrations. The order of effectiveness were: spermidine > N8-acetylspermidine > N1-acetylspermidine. Thus a decrease in efficiency was observed when the first order aminogroups of spermidine were blocked. The N-8amino-group of spermidine was less effective in inducing cleavage at AP-sites than the N1-amino-group. Among several proposed models ofpolyamine-DNA interactions, our results can best be explained by the model postulated by Liquori et al. [6]. © 1990.

引用

页码：389 / 393

页数：5

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