FLUORESCENT COLLOIDAL GOLD - CYTOCHEMICAL MARKER FOR FLUORESCENT AND ELECTRON-MICROSCOPY

被引：17

作者：

HORISBERGER, M

VONLANTHEN, M

机构：

[1] Nestlé Research Department, La Tour-de-Peilz, CH-1814

来源：

HISTOCHEMISTRY | 1979年 / 64卷 / 01期

关键词：

D O I：

10.1007/BF00493360

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The gold method was further developed for fluorescent microscopy. Gold granules (12 nm in size) were labelled with rhodamine conjugates of Concanavalin A and avidin. The fluorescent markers were used to mark cell wall mannan on the yeast Saccharomyces cerevisiae either by the one-step, or by the two-step method via a biotinyl derivative of ConA. By fluorescence or transmission electron microscopy, the two-step method was found to achieve a higher density of marking. © 1979 Springer-Verlag.

引用

收藏

页码：115 / 118

页数：4

相关论文

共 14 条

[1] PREPARATION OF FERRITIN-AVIDIN CONJUGATES BY REDUCTIVE ALKYLATION FOR USE IN ELECTRON-MICROSCOPIC CYTOCHEMISTRY [J].

BAYER, EA ;

SKUTELSKY, E ;

WYNNE, D ;

WILCHEK, M .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1976, 24 (08) :933-939

[2]

FAULK WP, 1971, IMMUNOCHEMISTRY, V8, P1081

[3] ADSORPTION OF HORSERADISH-PEROXIDASE, OVOMUCOID AND ANTIIMMUNOGLOBULIN TO COLLOIDAL GOLD FOR INDIRECT DETECTION OF CONCANAVALIN-A, WHEAT-GERM AGGLUTININ AND GOAT ANTIHUMAN IMMUNOGLOBULIN-G ON CELL-SURFACES AT ELECTRON-MICROSCOPIC LEVEL - NEW METHOD, THEORY AND APPLICATION [J].

GEOGHEGAN, WD ;

ACKERMAN, GA .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (11) :1187-1200

[4]

HEIZMANN H, 1974, P NATL ACAD SCI USA, V71, P3537

[5] SIMULTANEOUS LOCALIZATION OF AN HEPATIC BINDING-PROTEIN SPECIFIC FOR GALACTOSE AND OF GALACTOSE-CONTAINING RECEPTORS ON RAT HEPATOCYTES [J].

HORISBERGER, M ;

VONLANTHEN, M .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1978, 26 (11) :960-966

[6] COLLOIDAL GOLD, A USEFUL MARKER FOR TRANSMISSION AND SCANNING ELECTRON-MICROSCOPY [J].

HORISBERGER, M ;

ROSSET, J .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (04) :295-305

[7] LOCATION OF MANNAN AND CHITIN ON THIN-SECTIONS OF BUDDING YEASTS WITH GOLD MARKERS [J].

HORISBERGER, M ;

VONLANTHEN, M .

ARCHIVES OF MICROBIOLOGY, 1977, 115 (01) :1-7

[8]

HORISBERGER M, 1979, BIOL CELL, V36

[9] LYMPHOCYTE MEMBRANE LECTINS - DIRECT VISUALIZATION BY THE USE OF FLUORESCEINYL-GLYCOSYLATED CYTOCHEMICAL MARKERS [J].

KIEDA, C ;

ROCHE, AC ;

DELMOTTE, F ;

MONSIGNY, M .

FEBS LETTERS, 1979, 99 (02) :329-332

[10] NEW IMMUNOLATEX SPHERES - VISUAL MARKERS OF ANTIGENS ON LYMPHOCYTES FOR SCANNING ELECTRON-MICROSCOPY [J].

MOLDAY, RS ;

DREYER, WJ ;

REMBAUM, A ;

YEN, SPS .

JOURNAL OF CELL BIOLOGY, 1975, 64 (01) :75-88