LUMINOL CHEMILUMINESCENCE USING XANTHINE AND HYPOXANTHINE AS XANTHINE-OXIDASE SUBSTRATES

被引：49

作者：

RADI, R ^{[1
]}

RUBBO, H ^{[1
]}

THOMSON, L ^{[1
]}

PRODANOV, E ^{[1
]}

机构：

[1] UNIV REPUBL MONTEVIDEO,FAC MED,DEPT BIOCHEM,AV GEN FLORES 2125,CP 11800,MONTEVIDEO,URUGUAY

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 02期

关键词：

Chemiluminescence; Free radicals; Lucigenin; Luminol; Superoxide; Uric acid; Xanthine oxidase;

D O I：

10.1016/0891-5849(90)90084-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Luminol chemiluminescence induced by the xanthine or hypoxanthine-O2-xanthine oxidase system is analyzed and compared. Characteristics of the light emission curves were examined considering the conventional reaction scheme for the oxidation of both substrates in the presence of xanthine oxidase. The ratio of the areas of the light intensity versus time curves for hypoxanthine compared with xanthine is greater than that expected from the rate of superoxide production during substrate oxidation to uric acid. The O2{minus sign, dot below} to uric acid ratio for each substrate can account for differences in xanthine and hypoxanthine-supported light emission, since uric acid is a strong inhibitor of O2{minus sign, dot below}-dependent luminol chemiluminescence. These results are consistent with a free radical scavenging role for uric acid. A similar but weaker scavenging effect of xanthine may also contribute to the observed differences in chemiluminescent yields between both substrates. © 1990.

引用

页码：121 / 126

页数：6

共 24 条

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