LIQUID COLUMN CHROMATOGRAPHY OF OXO COMPOUNDS AS THEIR 2,4-DINITROPHENYLHYDRAZONES WITH SPECIAL REFERENCE TO GLUCOSE GLYCINE MAILLARD REACTION-PRODUCTS

Oxo compounds (2,4-dimtrophenylhydrazine-reactive) formed during the Maillard reaction between glucose and glycine were converted into 2,4-dinitrophenylhydrazones and were separated into five main fractions by reversed-phase chromatography on Separon SGX C18 using a methanoi-water gradient. Four of these fractions were homogeneous, as shown by thin-layer chromatography. One of the fractions still consisted of two zones, which, however, were easy to separate by preparative thin-layer chromatography on silica gel with tetrachloromethane-methanol-n-butanol (90:5:5, v/v/v) with dual development. The nature of the compounds separated is discussed. © 1990.