EXPRESSION AND CHARACTERIZATION OF AN ACTIVE AND THERMALLY MORE STABLE RECOMBINANT ANTIFREEZE POLYPEPTIDE FROM OCEAN POUT, MACROZOARCES-AMERICANUS, IN ESCHERICHIA-COLI - IMPROVED EXPRESSION BY THE MODIFICATION OF THE SECONDARY STRUCTURE OF THE MESSENGER-RNA

The cDNA clone coding for the ocean pout antifreeze polypeptide (AFP) was modified to improve translation of its mRNA in Escherichia coli. A recombinant AFP (rAFP), MetLys-AFP-Lys, was expressed successfully using the lambda-P(L) promoter, and constituted 1-2% of total bacterial proteins. The rAFP was purified to homogeneity from the soluble fractions of bacterial extracts. Its identity was confirmed by amino acid analysis, automated Edman degradation, immuno-blot and activity measurements. Although the rAFP is indistinguishable from the authentic AFP in its secondary structure, thermal hysteretic activity and the alteration of ice crystal structure, it is, however, thermally more stable (approximately 4.5-degrees-C increase in T(m)) and is more effective in inhibiting ice growth along the a-axis. These investigations indicate that the extra amino acids in rAFP significantly improve the thermal stability and ice-binding activity of the polypeptide.