A MULTIPLE LUMINESCENT PROCEDURE FOR THE DETECTION OF DIFFERENT PAPILLOMAVIRUSES ON DOT BLOTS

被引：3

作者：

CHIKHAOUI, Y ^{[1
]}

BALAGUER, P ^{[1
]}

TEROUANNE, B ^{[1
]}

BOUSSIOUX, AM ^{[1
]}

NICOLAS, JC ^{[1
]}

机构：

[1] INSERM,UNITE 58,60 RUE NAVACELLES,F-34090 MONTPELLIER,FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 150卷 / 1-2期

关键词：

HYBRIDIZATION; ENZYME; POLYMERASE CHAIN REACTION; OLIGONUCLEOTIDE; NONISOTOPIC;

D O I：

10.1016/0022-1759(92)90064-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a method based on the use of various luminescent systems for identification of several nucleic acid sequences on the same dot blots. In a simultaneous assay, the target DNA molecules were hybridized with different DNA probes. These probes were labelled with biotin or digoxigenin or directly coupled to enzymes such as glucose-6-phosphate dehydrogenase, peroxidase or alkaline phosphatase. After hybridization, these labels were detected by luminescent reactions using an amplified camera. Rapid detection and specific identification of pathogenic agents such as human papillomaviruses (HPV) could be performed in a single step by this procedure. Polymerase chain reaction was carried out using general primers and virus types were identified on dot blots using short HPV 11, 16 and 18,specific oligonucleotides.

引用

页码：51 / 56

页数：6

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