CLONING OF THE C-ALPHA CATALYTIC SUBUNIT OF THE BOVINE CAMP-DEPENDENT PROTEIN-KINASE

被引：18

作者：

WIEMANN, S

KINZEL, V

PYERIN, W

机构：

[1] GERMAN CANC RES CTR,DEPT PATHOCHEM,W-6900 HEIDELBERG,GERMANY

[2] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY

[3] GERMAN CANC RES CTR,PROJECT BIOCHEM CELL PHYSIOL,W-6900 HEIDELBERG 1,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1171卷 / 01期

关键词：

CAMP-DEPENDENT PROTEIN KINASE; C-ALPHA CATALYTIC SUBUNIT; CDNA; POLYMERASE CHAIN REACTION; (BOVINE);

D O I：

10.1016/0167-4781(92)90144-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bovine Calpha type catalytic subunit of the cAMP-dependent protein kinase was cloned. A partial cDNA was isolated from a bovine heart cDNA library. This clone contained 120 bp of the coding sequence and the entire 3' untranslated region of 1431 bp. The complete coding region was cloned by PCR amplification from total bovine heart and skeletal muscle RNA. The sequence of the 3' oligonucleotide was taken from the partial cDNA clone whereas the 5' oligonucleotide was chosen by comparison of sequences of published Calpha subunits from other species. In the deduced amino acid sequence there is one deviation from the published bovine Calpha protein sequence, aspartic acid 286 is exchanged by an asparagine. The Calpha mRNA was found to be expressed differentially in various bovine tissues.

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页码：93 / 96

页数：4

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