PHOTOLYSIS OF RHODOPSIN RESULTS IN DEPROTONATION OF ITS RETINAL SCHIFF-BASE PRIOR TO FORMATION OF METARHODOPSIN-II

被引：35

作者：

THORGEIRSSON, TE ^{[1
]}

LEWIS, JW ^{[1
]}

WALLACEWILLIAMS, SE ^{[1
]}

KLIGER, DS ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ,DEPT CHEM & BIOCHEM,SANTA CRUZ,CA 95064

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1992年 / 56卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1992.tb09738.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Absorption changes following photolysis of bovine rhodopsin in mildly sonicated membrane suspensions are monitored at 25-degrees-C. Difference spectra collected at 17 times between 1 muS and 75 ms following excitation are analyzed globally using singular value decomposition and non-linear least-squares fitting techniques. The results are not consistent with the simple scheme: Lumirhodopsin --> Metarhodopsin I reversible Metarhodopsin II, but indicate that an intermediate with a deprotonated Schiffs base is formed nearly simultaneously with Metarhodopsin I upon the decay of Lumirhodopsin.

引用

页码：1135 / 1144

页数：10

共 37 条

[1] PHOTOLYSIS INTERMEDIATES OF THE ARTIFICIAL VISUAL PIGMENT CIS-5,6-DIHYDRO-ISORHODOPSIN [J].

ALBECK, A ;

FRIEDMAN, N ;

OTTOLENGHI, M ;

SHEVES, M ;

EINTERZ, CM ;

HUG, SJ ;

LEWIS, JW ;

KLIGER, DS .

BIOPHYSICAL JOURNAL, 1989, 55 (02) :233-241

[2] DYNAMIC PROCESSES OF VISUAL TRANSDUCTION [J].

APPLEBURY, ML .

VISION RESEARCH, 1984, 24 (11) :1445-1454

[3]

BENNETT N, 1980, EUR J BIOCHEM, V111, P99

[4] EARLY PHOTOLYSIS INTERMEDIATES OF THE ARTIFICIAL VISUAL PIGMENT 13-DEMETHYLRHODOPSIN [J].

EINTERZ, CM ;

HUG, SJ ;

LEWIS, JW ;

KLIGER, DS .

BIOCHEMISTRY, 1990, 29 (06) :1485-1491

[5]

EMRICH HM, 1974, Z NATURFORSCH C, VC 29, P577

[6] REVERSAL OF PHOTORECEPTOR BLEACHING AND ADAPTATION BY MICROSECOND FLASHES [J].

ERNST, W ;

KEMP, CM .

VISION RESEARCH, 1979, 19 (04) :363-365

[7]

Haken H, 1978, SYNERGETICS

[8] THE STRUCTURE OF BACTERIORHODOPSIN AND ITS RELEVANCE TO THE VISUAL OPSINS AND OTHER 7-HELIX G-PROTEIN COUPLED RECEPTORS [J].

HENDERSON, R ;

SCHERTLER, GFX .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1990, 326 (1236) :379-&

[9]

HENRY ER, 1992, METHOD ENZYMOL, V210, P129

[10]

HOFMANN KP, 1986, PHOTOBIOCH PHOTOBIOP, V13, P309

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