TRYPSIN DIGESTION OF ARGINASE - EVIDENCE FOR A STABLE CONFORMATION MANGANESE DIRECTED

被引：13

作者：

DIEZ, AM ^{[1
]}

CAMPO, ML ^{[1
]}

SOLER, G ^{[1
]}

机构：

[1] UNIV EXTREMADURA,FAC VET,DEPT BIOQUIM & BIOL MOLEC & GENET,E-10071 CACERES,SPAIN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1992年 / 24卷 / 12期

关键词：

D O I：

10.1016/0020-711X(92)90288-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Controlled tryptic digestion of native arginase from mt fiver suggests that Mn2+ promotes a stable conformation as shown by the following features. 2. An 18-fold increase in the half-life of arginase activity in the presence of Mn2+ is produced. 3. The stability of subunit B of arginase is increased in the presence of Mn2+ as revealed by SDS-PAGE during the time-course of trypsin cleavage. 4. The different digestion products of arginase with and without Mn2+ appearing during the time-course of tryptic treatment. 5. Different activity/bands protein ratio at any time of the tryptic digestion in the incubation mixtures, with and without Mn2+, are apparent.

引用

页码：1925 / 1932

页数：8

共 29 条

[1]

BAUTISTA J, 1992, IN PRESS BIOCH BIOPH

[2] EFFECT OF NADP+ AND NADPH ON CONTROLLED TRYPTIC CLEAVAGE OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM DICENTRARCHUS-LABRAX (BASS) LIVER [J].

BAUTISTA, JM ;

GARRIDO, A ;

SOLER, G .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1988, 16 (05) :903-904

[3]

Bergmeyer H., 1986, METHOD ENZYMAT AN, P119

[4]

BOND JS, 1983, J BIOL CHEM, V258, P8004

[5]

BOND JS, 1986, MANGANESE METABOLISM, P239

[6]

Boyer PD, 1942, J BIOL CHEM, V143, P417

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] A CORRELATION BETWEEN PROTEIN THERMOSTABILITY AND RESISTANCE TO PROTEOLYSIS [J].

DANIEL, RM ;

COWAN, DA ;

MORGAN, HW ;

CURRAN, MP .

BIOCHEMICAL JOURNAL, 1982, 207 (03) :641-644

[9]

DIEZ A, 1990, THESIS

[10]

DUX L, 1983, J BIOL CHEM, V258, P111

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