SPONTANEOUS CALCIUM OSCILLATIONS IN CLONAL ENDOCRINE PANCREATIC GLUCAGON-SECRETING CELLS

被引：13

作者：

BODE, HP

YULE, DI

FEHMANN, HC

GOKE, B

WILLIAMS, JA

机构：

[1] UNIV MARBURG,CLIN RES UNIT GASTROINTESTINAL ENDOCRINOL,D-35033 MARBURG,GERMANY

[2] UNIV MICHIGAN,SCH MED,DEPT PHYSIOL,ANN ARBOR,MI

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Measurements of the cytosolic calcium concentration in single cells of the clonal endocrine pancreatic glucagon-secreting cell line INR1 G9 revealed the existence of spontaneous calcium oscillations in 20 - 70 % of these cells. Inhibition of these spontaneous oscillations by thapsigargin as well as the phospholipase C inhibitor U 73122 demonstrated involvement of calcium release from intracellular stores, probably mediated by a high basal activity of phospholipase C. Removal of extracellular calcium but not the L-type calcium channel antagonists verapamil or nifedipine terminated the spontaneous oscillations, suggesting that calcium influx by a pathway distinct from L-type channels contributed to the oscillations. Similar spontaneous calcium oscillations could be the pacemaker of pulsatile glucagon release in endocrine pancreatic A-cells. (C) 1994 Academic Press, me.

引用

页码：435 / 440

页数：6

共 21 条

[1] THE PHORBOL ESTER RECEPTOR - A PHOSPHOLIPID-REGULATED PROTEIN-KINASE [J].