VASOACTIVE INTESTINAL POLYPEPTIDE GENE-EXPRESSION IS CHARACTERISTICALLY HIGHER IN OPOSSUM GASTROINTESTINAL SPHINCTERS

被引：5

作者：

BANDYOPADHYAY, A ^{[1
]}

CHAKDER, S ^{[1
]}

LYNN, RB ^{[1
]}

RATTAN, S ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT MED, DIV GASTROENTEROL & HEPATOL, PHILADELPHIA, PA 19107 USA

来源：

GASTROENTEROLOGY | 1994年 / 106卷 / 06期

关键词：

D O I：

10.1016/0016-5085(94)90399-9

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: Vasoactive intestinal polypeptide (VIP) has been suggested to be an inhibitory neuro-transmitter in the sphincteric and nonsphincteric smooth muscles of the gut. However, the relative gene expression of VIP in these functionally diverse regions is not known. Methods: The gastrointestinal smooth muscle sphincters of opossums were excised from the adjoining nonsphincteric smooth muscles. RNAs were isolated and subjected to blot hybridizations with VIP complementary DNA probe. Relative expression of VIP gene was quantitated using the densitometric scanning of the VIP messenger RNA (mRNA) transcripts. The cellular specificity of VIP gene expression was investigated in cultures of neuroblastoma cells and myenteric plexuses and compared with those of the smooth muscle cells. Results: The data showed higher levels of VIP mRNA in the sphincteric than the adjoining nonsphincteric tissues. VIP mRNA were found in significantly higher amounts in the myenteric neurons and neuroblastoma cells than in the smooth muscle cells. Conclusions: VIP gene expression was significantly higher in the sphincteric smooth muscle regions than in the nonsphincteric regions of the gut. The studies provide further evidence for the role of VIP in neurotransmission of the gut. © 1994.

引用

页码：1467 / 1476

页数：10

共 54 条

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