INDUCTION OF HEAT-SHOCK PROTEIN-70 AND NUCLEOLIN AND THEIR INTRACELLULAR-DISTRIBUTION DURING EARLY-STAGE OF LIVER-REGENERATION

被引:26
作者
KONISHI, T
KARASAKI, Y
NOMOTO, M
OHMORI, H
SHIBATA, K
ABE, T
SHIMIZU, K
ITOH, H
HIGASHI, K
机构
[1] UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT BIOCHEM,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN
[2] UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT SURG 1,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN
[3] UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT BIOL MOLEC,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN
[4] ISHINOMAKI SENSHU UNIV,DEPT BIOTECHNOL,ISHINOMAKI,MIYAGI 986,JAPAN
关键词
G(1) PHASE; HSP70; NUCLEAR LOCALIZATION; NUCLEOLIN; REGENERATING LIVER (RAT);
D O I
10.1093/oxfordjournals.jbchem.a124840
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our previous work demonstrated a simultaneous induction of Hsp70 and nucleolin mRNA during the prereplicative stage of hepatocytes after partial hepatectomy [Ohmori, H. et al. (1990) Exp. Cell Res. 189, 227-232]. In the present study, changes of intracellular localization of these proteins were examined. Nucleolin, which mainly localized in the nucleus, increased with time and accumulated in the nucleolus around 12-18 h after partial hepatectomy. Hsp70 protein also increased slightly around 6-12 h after the operation. In accordance with this increase, immunohistochemical staining revealed that almost all nuclei of hepatocytes became Hsp70-positive, although Hsp70 was seen to be dispersed throughout the nucleoplasm and nucleolus at all times examined. Next, we isolated a cDNA clone of ribosomal protein (S-17) and examined its behavior. Induction of S-17 mRNA was observed to be essentially similar to that of nucleolin mRNA in regenerating rat liver, although inductions of histone H2A and H4 occurred at a later time, that is, in parallel with DNA synthesis. Furthermore, we observed a simultaneous induction of Hsp70 and nucleolin mRNA by serum-stimulation after serum-depletion in HeLa and IAR-20 (rat) culture cells. These results suggest that the induction of Hsp70, in addition to nucleolin, was not fortuitous but may be involved in the early events of liver regeneration.
引用
收藏
页码:1170 / 1177
页数:8
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